Immunomodulatory effect of beta-glucan derived from Saccharomyces cerevisiae strains / 동물의과학연구지

The aim of this study was to evaluate immunopotentiating activities of beta-glucan derived from Saccharomyces (S.) cerevisiae and to select new strains having possibility as an immune-enhancing substance. We examined SB20 strains derived from commercial product as a control, and extracted beta-glucans from the four strains of S. cerevisiae. RAW264.7 macrophages were treated with heat-killed yeasts, beta-glucans, and lipopolysaccharide (LPS). The production of nitric oxide (NO) and cytokines such as TNF-alpha and IL-1beta were then quantified. When macrophages were induced directly by in vitro addition of beta-glucan, little production of NO and IL-1beta was observed. When pretreated with strong stimulants, i.e., LPS, most yeasts showed down-modulation of NO and IL-1beta production. However, TNF-alpha secretion was triggered by beta-glucans and even more increased by the mixture effect of LPS and beta-glucans. In particular, S6 strain induced TNF-alpha secretion more than other strains. Therefore, we can conclude that the S6 strain has possibility as an immune-enhancing substance.

Immuno-enhancing Effects of Lactobacillus salivarius JWS 58 and Lactobacillus plantarum JWS 1354 isolated from duck / 대한수의학회지

Lactobacillus salivarius JWS 58 (JWS 58) and Lactobacillus plantarum JWS 1354 (JWS 1354) are isolated from duck intestine and have ability to produce bacteriocin. The objective of this study was to evaluate the immunomodulatory effects of JWS 58 and JWS 1354. The nitric oxide (NO) and cytokines (IL-1beta and TNF-alpha) were measured in C57BL/6 mouse peritoneal macrophages to determine immune enhancing effects of JWS 58 and JWS 1354. A Listeria (L.) monocytogenes challenge mice model was used to evaluate immune enhancement ability of JWS 58 and JWS 1354 in vivo. The results showed that JWS 58 and JWS 1354 increased the production of NO or cytokines by peritoneal macrophages and that oral administration of viable probiotic strains in mice elicited the immuno-modulatory effect upon L. monocytogenes challenge. JWS 1354 showed stronger immune enhancing effects than JWS 58. Collectively, this study demonstrated that Lactobacillus strain JWS 58 and JWS 1354 possess immune enhancing effect. Furthermore, two stains are expected to use feed supplement to prevent diseases by pathogenic bacteria through releasing bacteriocin and enhancing host immune responses in animal.