Résumé
The anti-tumor effect of monocyte-derived DC (MoDC) vaccine was studied in lung cancer model with feasible but weak Ag-specific immune response and incomplete blocking of tumor growth. To overcome this limitation, the hematopoietic stem cell-derived DC (SDC) was cultured and the anti-tumor effect of MoDC & SDC was compared in mouse lung cancer minimal residual model (MRD). Therapeutic DCs were cultured from either CD34+ hematopoietic stem cells with GM-CSF, SCF and IL-4 for 14 days (SDC) or monocytes with GM-CSF and IL-4 for 7 days (MoDC). DCs were injected twice by one week interval into the peritoneum of mice that are inoculated with Lewis Lung Carcinoma cells (LLC) one day before the DC injection. Anti-tumor responses and the immune modulation were observed 3 weeks after the final DC injection. CD11c expression, IL-12 and TGF-beta secretion were higher in SDC but CCR7 expression, IFN-gamma and IL-10 secretion were higher in MoDC. The proportion of CD11c+CD8a+ cells was similar in both DC cultures. Although both DC reduced the tumor burden, histological anti-tumor effect and the frequencies of IFN-gamma secreting CD8+ T cells were higher in SDC treated group than in MoDC. Conclusively, although both MoDC and SDC can induce the anti-tumor immunity, SDC may be better module as anti-tumor vaccine than MoDC in mouse lung cancer.
Sujets)
Animaux , Souris , Carcinome pulmonaire de Lewis , Cellules dendritiques , Facteur de stimulation des colonies de granulocytes et de macrophages , Cellules souches hématopoïétiques , Interleukine-10 , Interleukine-12 , Interleukine-4 , Poumon , Tumeurs du poumon , Monocytes , Péritoine , Lymphocytes T , Facteur de croissance transformant bêta , Charge tumoraleRésumé
BACKGROUND: Dendritic cell (DC)-based cancer immunotherapy is studied for several years. However, it is mainly derived from autologous PBMC or leukapheresis from patient, which has limitations about yield and ability of DC production according to individual status. In order to solve these problems, inquiries about allogeneic DCs are performed but there are no preclinical trial answers for effect or toxicity of allogeneic DC to use for clinical trial. In this study, we compared the anti-tumor effect of allogeneic and autologous DCs from mouse bone marrow stem cells in mouse metastatic melanoma model. METHODS: B16F10 melanoma cells (5 x 10(4)/mouse) were injected intravenously into the C57BL/6 mouse. Therapeutic DCs were differentiated from autologous (C57BL/6: CDC) or allogeneic (B6C3F1: BDC) bone marrow stem cells with GM-CSF, SCF and IL-4 for 13days and pulsed with B16F10 tumor cell lysate (Blys) for 18hrs. DC intra-peritoneal injections began on the 8th day after the tumor cell injection by twice with one week interval. RESULTS: Anti-tumor response was observed by DC treatment without any toxicity especially in allogeneic DC treated mice (tumor burden score: 2.667+/-0.184, 2.500+/-0.463, 2.000+/-0.286, 1.500+/-0.286, 1.667+/-0.297 for saline, CDC/unpulsed-DC: U-DC, CDC/Blys-DC, BDC/U-DC and BDC/Blys-DC, respectively). IFN-gamma secretion was significantly increased in allogeneic DC group stimulated with B16F10 cell lysate (2,643.3+/-5,89.7, 8,561.5+/-2,204.9. 6,901.2+/-141.1 pg/1 x 10(6) cells for saline, BDC/U-DC and BDC/Blys-DC, respectively) with increased NK cell activity. CONCLUSION: Conclusively, promising data was obtained that allogeneic DC can be used for DC-based cancer immunotherapy.
Sujets)
Animaux , Humains , Souris , Moelle osseuse , Cellules dendritiques , Facteur de stimulation des colonies de granulocytes et de macrophages , Immunothérapie , Interleukine-4 , Cellules tueuses naturelles , Leucaphérèse , Mélanome , Métastase tumorale , Cellules souchesRésumé
Spindle cell hemangioendothelioma was first described in 1986 to characterize a particular angiomatous lesion which shows different clinical features, however, it has recently been designated as spindle cell hemangioma (SCH) due to its benign nature. Microscopically, this peculiar vascular tumor consists of cavernous blood vessels intermixed with solid areas predominantly composed of spindle cells, resembling Kaposi sarcoma. A 39-year-old woman presented with a bean-sized, tender, bluish nodule on the pulp of her right thumb. The 1 year old lesion had increased slowly in size over this time. Histologic examination of the lesion showed that it was composed of large irregularly-dilated, thin-walled cavernous blood spaces containing organizing thrombi, numerous red blood cells, and a spindle cell mass which was partially connected with the vessel wall and invaded the stroma. A diagnosis of SCH was made based on histologic and immunohistochemical findings. After the lesion was totally excised, there has been neither recurrence nor occurence of new lesions to date.
Sujets)
Adulte , Femelle , Humains , Vaisseaux sanguins , Diagnostic , Érythrocytes , Hémangioendothéliome , Hémangiome , Récidive , Sarcome de Kaposi , PouceRésumé
A glomus tumor is a benign neoplasm of neuromyo-arterial glomus, composed of vascular channels surrounded by proliferating glomus cells and nerve fibers. Occasionally it displays unusual histopathologic features such as a large size, deep location, infiltrative growth, mitotic activity, nuclear pleomorphism, or necrosis. It can be diagnosed as an "atypical" glomus tumor, including malignant glomus tumor, glomus tumor of uncertain malignant potential, symplastic glomus tumor, or glomangiomatosis. Glomangiomatosis and symplastic glomus tumors reveal benign biologic behavior. We report a typical case of a symplastic glomus tumor, showing only marked nuclear atypia, compared with a classical glomus tumor.
Sujets)
Tumeur glomique , Nécrose , NeurofibresRésumé
Mixed tumor of the skin, so called chondroid syringoma, is a benign epithelial neoplasm with glandular or ductal differentiation surrounded by myxoid stroma. It is most frequently located on the head and neck and presents as an asymptomatic, firm, subcutaneous nodule. Mixed tumor with follicular, sebaceous and apocrine differentiation is considered as an expression of the common embryologic origin of elements of the folliculo-sebaceous-apocrine unit. We report a case of mixed tumor of the skin with follicular, sebaceous and apocrine differentiation.
Sujets)
Adénome pléomorphe , Tête , Cou , Tumeurs épithéliales épidermoïdes et glandulaires , PeauRésumé
Nodular fasciitis represents reactive fibroblastic or myofibroblastic proliferative lesions which may be misdiagnosed as sarcomas due to the rich cellularity, mitotic activity and variant morphologic pattern. It arises in the subcutaneous tissue, skeletal muscles, and fascia. Uncommon clinical and pathological variants of nodular fasciitis, such as intradermal, intravascular, cranial, ossifying, parosteal and proliferative fasciitis, have been described. We present a 23-year-old man with a one year history of a 2x1.5cm sized hard tender nodule on the scalp which is partially depressed at the margin. Histological examination revealed clusters of spindle cells in a myxoid background, chronic inflammatory cells and extravasated red cells in the dermis. Immunohistochemically, the spindle cells showed positivity of vimentin or smooth muscle actin, and negativity of desmin or S-100 protein. The staining results support its myofibroblastic origin. There is no involved bony lesion on the brain computed tomography (CT). Therefore we report a rare case of intradermal fasciitis arising on the scalp.