Résumé
The effects of dimethylsulfoxide [DMSO] and ethanol [used as solvents for hormones] on the rate of protein synthesis in washed ram spermatozoa was examined. Estradiol 17-beta was tested for the possible effects on the metabolism of washed ram spermatozoa when incubated at 37°C in the media used with or without glucose. The results showed that DMSO and ethanol suppressed protein synthesis in washed ram spermatozoa. Ethanol [0.1%] caused the least degree of inhibition in protein synthesis. Estradiol 17-beta stimulated protein synthesis in media containing glucose. Estradiol 17-beta, however, failed to stimulate protein synthesis in media deprived of glucose. This emphasise the importance of utilizing glucose as a nutrient in the media used by sperm cells