Treatment of cutaneous leishmaniasis with methanol extract of Allium sativa [garlic], Azadirachta indica [Neem] and Accacia nilotica [Garad] in Gezira Central of Sudan

To assess the in vivo treatment of cutaneous leishmaniasis using certain medicinal agents and non medicinal agents in Gezira State, Sudan. To evaluate the in vivo anti-lesihmanial activity of certain plant extracts that used traditionally in Sudan [Azadirachta indica [neem], Accacia nilotica [garad], Allium sativa [garlic, Thoum]]. A total of 72 cases with cutaneous leishmanisis were studied in Wad Madani Dermatology Teaching Hospital [September 1999 - December 2000]. They were from different parts of the Gezira state. The patients were interviewed and examined followed a special designed questionnaire. The cases showed different types of lesions with different duration and appeared in different areas of the body. The cases divided equally into six main groups [12 for each] for treatment with different agents including medicinal agents and plant methanol extracts

Differential display analysis of mRNAs in chronic myelogenous leukaemia

Analysis of expressed mRNAs with differential display-polymerase chain reaction [DD-PCR] is a powerful tool for the characterization of genes involved in malignant pathways and might identify markers for different phases of chronic myelogenous leukaemia [CML]. We examined the presence of BCR-ABL transcripts in 25 CML patients in either the chronic phase or blast crisis. We then analysed the expression of leukocytic RNA transcripts in CML phases. DD-PCR technique was used to examine CML cases with BCR-ABL in comparison with CML cases lacking detectable BCR-ABL transcripts. Our results support the use of differential display not only for characterization of the CML differentially expressed genes but also to locate patterns that can be implemented as valuable fingerprints for each phase of CML