Antibacterial effect of an antibacterial adhesive system, and a resin modified glass ionomer liner

To evaluate the antibacterial effect of an antibacterial adhesive system and a resin modified glass ionomer liner against Streptococcus mutans. The antibacterial effect of the tested materials was evaluated using the agar disc diffusion test. Filter paper discs loaded with the tested materials were applied to agar plates previously inoculated with Streptococcus mutans. After incubation the inhibitory effect of the materials were evaluated by measuring the size of the inhibition zones. Resulted showed that both of the tested materials have a strong antibacterial effect. The primer of the anti bacterial adhesive system has significantly high antibacterial effect than the resin modified glass ionomer [Vitribond] [P < .001]. The use of the antibacterial adhesive system for bonding of composites provides higher antibacterial effect than the use of glass ionomer liner with composite resin restorations

Assessment of the nanoleakage of an antibacterial adhesive system and a resin modified glass ionomer liner

To evaluate the performance of an antibacterial self-etch adhesive systems and a resin modified glass ionomer liner by examining the resin-dentin interface and assessing the nanoleakage. The occlusal surfaces of 20 sound human premolars were ground and divided into two groups one coated with an antibacterial self etch adhesive system Clearfil Protect Bond and the other group was treated with a resin modified glass ionomer liner Vitribond. All teeth were coated with a thin layer of composite. The restored teeth were immersed in physiologic saline for 24 h. Then they were subjected to further immersion in 50% ammoniacal silver nitrate solution for 18 h and soaked in photo-developing solution for 6h. All teeth were sectioned in a bucco-lingual direction. The cut surfaces were polished, followed by 6N HCL application for 45s. All specimens were examined using Environmental Scanning Microscope to characterize the hybrid layer and evaluate the nanoleakage. A thin hybrid layer about 2-3 micro m wide was found at the interface of Clearfil Protect Bond with minimal silver deposition detected by the EDX analysis. Vitribond showed an interaction zone at the dentin interface with heavy deposition of silver granules. Clearfil Protect bond showed minimal degree of nanoleakage. Clearfil Protect Bond provides a hybrid layer at the resin/dentin interface with minimal degree of associated nanoleakage