Metabolite profiling and in-vitro evaluation of cytotoxic efficacy of Psychotria dalzellii Hook.f. on MCF-7 and L929 cell lines

In the current study Psychotria dalzellii Hook.f. stem-bark samples were procured from Joida, Western Ghats, Karnataka, India. air-dried and subjected to Soxhlet extraction. The obtained extracts were screened for preliminary phytochemicals which resulted in the existence of flavonoids, alkaloids, glycosides, phenols, tannins, steroids, triterpenoids, saponins, carbohydrates and lignins. Subsequently, the ethanolic stem bark extracts were subjected to GC-MS analysis to quantitatively estimate the phytoconstituents. It reveals the presence of 20 bioactive chemicals. Among them, 2-Furancarboxaldehyde, 5-(hydroxymethyl)- (59.26%); p-Mesyloxyphenol (10.68%); 2-Furaldehyde diethyl acetal (4.54%); 4H-Pyran-4-one, 2,3-dihydro-3,5-dihydroxy-6-methyl (4.11%) were noted to be the principal compounds detected. Furthermore, in-vitro cytotoxic activities on MCF-7 and L929 cell lines were determined by 3-[4,5-dimethylthiazol-2-yl]-2,5 diphenyl tetrazolium bromide assay. The extract exhibited considerable cytotoxic activity towards MCF-7 cell lines at higher concentrations with an IC50 value of 440.82 µg/ml and it showed very less toxicity towards L929 normal cell lines. Which indicates its potential as a possible source of bioactive constituents for medicinal and pharmaceutical exploration.

Evaluation of In-vitro anti-inflammatory and antibacterial potential of methanolic leaf extracts of Elytranthe parasitica (L.) Danser (Loranthaceae) – A Hemiparasitic Angiosperm

Elytranthe parasitica (L.) Danser is a hemiparasitic angiosperm shrub commonly called Macrosolen parasiticus and it is a member of the family Loranthaceae. The members of Loranthaceae are utilized globally in conventional medicine to cure a variety of ailments. The current study aimed to assess the qualitative screening of Elytranthe parasitica for its secondary metabolites and also In-vitro evaluation of anti-inflammatory and antibacterial potential. The fresh leaf samples were collected and extracted with the help of a soxhlet extractor. The in-vitro anti-inflammatory potential was determined by protein denaturation assay and HRBC membrane stabilization assay, and evaluation of antibacterial properties by agar well diffusion assay. The results of the qualitative evaluation of phytoconstituents revealed a wide range of secondary metabolites. The methanolic leaf extracts of Elytranthe parasitic exhibited significant protein denaturation activity of (82.46 ± 1.37)% at 250 µg ml-1 concentration and showed significant membrane stabilization activity (65.57±2.60)% at 500 µg ml-1 concentration. It also shows good membrane stabilizations activity shows considerable antibacterial activity with the maximum inhibition zone against Xanthomonas campestris (18.16±0.44 mm) followed by Escherichia coli (15.33±0.88 mm), Staphylococcus aureus (11.16±0.44), and Salmonella typhi (10.66±0.66 mm) at higher concentrations. To conclude, it was found that Elytranthe parasitica (L.) Danser has a variety of secondary metabolites that have potent anti-inflammatory and antibacterial properties based on the experiments carried out.