1.
Indian J Exp Biol
;
1997 Jan; 35(1): 86-8
Article
Dans Anglais
| IMSEAR
| ID: sea-61869
Résumé
In the present study, a rapid and simple colorimetric technique has been described to determine the presence of bacteria in tissue culture medium used in animal cell culture. The microplate assay is based on utilization of MTT [3(4,5-dimethylthiazol-2yl)-2,5-diphenyltetrazolium bromide] by bacteria resulting in formation of formazan crystals which can be measured colorimetrically. Contaminated medium, a standard gram-negative and gram-positive bacteria produce formazan from MTT which is related to the bacterial load. The assay has utility in screening tissue culture reagents to detect the presence of bacteria.
Sujets)
Bactéries/isolement et purification , Techniques bactériologiques/méthodes , Cristallisation , Milieux de culture/analyse , Contamination de médicament , Formazanes/métabolisme , Sels de tétrazolium/métabolisme , Thiazoles/métabolisme
2.
Indian J Exp Biol
;
1993 May; 31(5): 480-1
Article
Dans Anglais
| IMSEAR
| ID: sea-56727