Astrodaucus persicus as a new source of bioinsectisides against malaria vector, Anopheles stephensi

Objective To determine the larvicidal activities of petroleum ether, chloroform, ethyl acetate and methanol fractions of roots and fruits extracts of Astrodaucus persicus from Apiaceae family against malaria vector, Anopheles stephensi (An. stephensi). Methods Twenty five third instar larvae of An. stephensi were exposed to various concentrations (10–160 g/L) of fractions and were assayed according to World Health Organization protocol. The larval mortality was calculated after 24 h treatment. Results Among tested fractions, the highest larvicidal efficacy was observed from ethyl acetate fraction of fruits extract with 50% and 90% mortality values (LC

Astrodaucus persicus as a new source of bioinsectisides against malaria vector, Anopheles stephensi

OBJECTIVE@#To determine the larvicidal activities of petroleum ether, chloroform, ethyl acetate and methanol fractions of roots and fruits extracts of Astrodaucus persicus from Apiaceae family against malaria vector, Anopheles stephensi (An. stephensi).@*METHODS@#Twenty five third instar larvae of An. stephensi were exposed to various concentrations (10-160 g/L) of fractions and were assayed according to World Health Organization protocol. The larval mortality was calculated after 24 h treatment.@*RESULTS@#Among tested fractions, the highest larvicidal efficacy was observed from ethyl acetate fraction of fruits extract with 50% and 90% mortality values (LC and LC) of 34.49 g/L and 108.61 g/L, respectively. Chloroform fraction of fruits extract was the second larvicidal sample with LC of 45.11 g/L and LC of 139.36 g/L. Petroleum ether fractions of fruits and roots and methanol fraction of fruits showed moderate toxicity against An. stephensi.@*CONCLUSIONS@#Astrodaucus persicus is a potential source of valuable and natural larvicidal compounds against malaria vector, An. stephensi and can be used in mosquitoes control programs as an alternative to synthetic insecticides.

Antimicrobial activities of three medicinal plants and investigation of flavonoids of Tripleurospermum disciforme

Rosa damascena, Tripleurospermum disciforme and Securigera securidaca were used as disinfectant agents and for treatment of some disease in folk medicine of Iran. The antimicrobial effects of different fractions of seeds extract of S. securidaca, petals extract of R. damascena and aerial parts extract of T. disciforme were examined against some gram positive, gram negative and fungi by cup plate diffusion method. The petroleum ether and chloroform fractions of S. securidaca showed antibacterial activities against Staphylococcus aureus and Pseudomonas aeruginosa, while its methanol fraction had no antibacterial effects. R. damascena petals extract demonstrated antibacterial activities against Bacillus cereus, Staphylococcus epidermidis, S. aureus and Pseudomonas aeruginosa. T. disciforme aerial parts extract exhibited antimicrobial effects only against S. aureus and S. epidermidis. None of the fractions had any antifungal activities. Therefore, present study confirmed utility of these plants as disinfectant agents. Six flavonoids were isolated from T. disciforme: Luteolin, Quercetin-7-O-glucoside, Kaempferol, Kaempferol-7-O-glucoside, Apigenin and Apigenin-7-O-glucoside. The flavonoids and the antimicrobial activity of T. disciforme are reported for the first time

Bioactivity of major components from the seeds of bunium persicum [boiss.] fedtch

Zire Kermani [Bunium persicum] is an Iranian plant which is commonly used as antispasmodic, carminative, anti obesity and lactogage. The essential oil and different extracts of the seeds of this plant were evaluated for antioxidant activity by three complementary methods: DPPH assay, beta-carotene bleaching and ammonium thiocyanate methods. The oil and methanolic extract of B. persicum exhibited the highest antioxidant activity with IC50=23.4 +/- 1.6 and 45.7 +/- 3.6 micro gmL-1 in DPPH assay and the most inhibition of beta-carotene oxidation and lipid peroxidation. The GC/MS analysis of the essential oil of B. persicum indicated the gamma-Terpinene [46.1%] and cuminaldehyde [15.5%] as the major components of the oil. Furthermore the active methanol extract of the plant was fractioned by column chromatography to afford several fractions, of which in more purification, kaempferol, caffeic acid and p-coumaric acid were found to be the antioxidant components of this extract. These results confirm the in vitro antioxidant and radical scavenging activity of the essential oil and methanolic extract of B. persicum, which warranty the use of the plant in many traditional uses