Role of 5HT in the modification of intestinal motility, in vitro study

To determine with the mechanism of action involved in the therapeutic potential of serotonin and its blocker on gastrointestinal motility. The standard method was used for obtaining the longitudinal and circular muscles strip of rabbit ileum for in vitro studies. Each muscle strip was exposed to serotonin and its blocker and the result obtained was recorded on polygraph apparatus. The effects were recorded in vice versa fashion i.e. agonist v/s antagonist and antagonist v/s agonist on longitudinal and circular muscle strip separately. Serotonin had depressant effect on the force of contraction. On addition of antagonist in the presence of agonist, the effects were increased. Longitudinal muscle showed more pronounced effect i.e. 52.7% with methysergide in comparison to circular muscle, which was 15.6%. Circular muscle showed reduction in the force of contraction with serotonin, which was increased on addition of antagonist, but still below the level of base line contraction. Serotonin when given from external source in vitro, decreased the force, however, there was minimal increase in the rate of contraction. Hence, serotonin decreases the intestinal motility giving an impression of having antispasmodic effect. The results of this study can be utilized in the development of new drug related to G.I. motility mediated through 5HT receptors

Abdominal tuberculosis: a varied presentation

Abdominal Tuberculosis presents to the general surgeon in one of the three forms: 1, intestinal obstruction 2, tuberculous peritonitis 3, abdominal mass. In this report, patients presenting with intestinal obstruction [Acute or sub acute], over a two year period at the Department of General Surgery, Shaikh Zayed Hospital, Lahore, 30 cases were found to have intestinal tuberculosis out of a total 100 cases studied. All patients were diagnosed on exploration with hisopathological evidence of tuberculosis and/or demonstration of A.F.B. in the lesion. The mean age was 34.9 years, male to female ratio 2: 1. 86% of the patients had primary intestinal tuberculosis whereas 14% were considered to be suffering from secondary intestinal tuberculosis. Majority of the patients [66%], had single multiple strictures involving the triminal ileum and ileocaecal region, 20% of the patients were found to have mass in the ileocaecal region and perforation was seen in 14% of the cases. Primary resection of the tuberculous lesion was carried out in 28 patients. Stricturoplasty was performed in the remaining 2 patients. Hospital mortality was zero and major post operative complications were noted in 8% of the cases. The patients received standard anti-tuberculosis treatment for a period of 12 months, follow up record was available on only 26 patients. All 26 patients had completed the anti-tuberculosis medication without any ill effects. No patient had presented with recurrent problem of obstruction

Abdominal tuberculosis, a varied presentation

Abdominal Tuberculosis presents to the general surgeon in one of the three forms: I, Intestinal Obstruction 2, Tuberculous peritonitis 3, abdominal mass. In a review of patients presenting with intestinal obstruction [Acute or sub acute], over a 2 years period at Shaikh Zayed hospital, Lahore, 30 cases were found to have intestinal tuberculosis out of a 100 of intestinal obstruction studied. All patients were diagnosed on exploration with histopathological evidence of tuberculosis and or demonstration of A.F.B in the lesion. The mean age was 34.9 years, male to female ration 2:1; 86% of the patients had primary intestinal tuberculosis whereas 14% are considered to be suffering from secondary intestinal tuberculosis. The majority of the patient [66%] had single on multiple strictures involving the terminal ileum and ileocecal region, 20% of the patients were found to have mass in the ileocecal region and perforation was seen in 14% of the cases. Primary resection of the tuberculous lesion was carried out in 28 patients. Hospital mortality was zero and major post op. complications were noted in 8%. All patients received standard anti-tuberculosis treatment for a period of 12 months. The follow up record is available on 26 patents. All 26 patients have so far completed the anti-tuberculosis medication without any ill effects. No patient has so far presented with a recurrent problem of obstruction