RÉSUMÉ
Aim of the study: This study was undertaken to investigate the potential role of exercise to attenuate remote pulmonary damage fallowing rat hindlimb ischemia reperfusion injury [I/R]
Materials and methods: forty five adult male rats were divided into three groups [15 animals each]: The sham group, I/R group and the exercised group [E + I/R group]. The rats were made to swim for 1 h, five vines a week, for 4 weeks before I/R. Bilateral hindlimb ischemia was induced by application of rubber bands above the greater trochanter for 3 h. Following 3 h of reperfusion, blood samples were taken for determination of arterial partial pressure of oxygen [PaO[2]], arterial partial pressure of carbon dioxide [PaCO[2]], malondialdehyde [MDA], superoxide disniutase [SOD], tumor necrosis-alpha [TNF-alpha] and interleukin-6 [IL-6]. The animals were scarified and right lung were prepared for histological evaluation and immunohistochemical analysis for caspas-3 and the left lung used for lung tissue wet- to-dry weight with [W/D]
Results: In I/R group, the PaO[2] and SOD levels were decreased, plasma levels of MDA, TNF- alpha and IL-6 and W/D ratio were significantly increased when compared to sham group, there was prominent thickening of the interalveolar sepia and hypertrophy of the smooth muscles around the bronchioles. Interstitial edema and capillary congestion can he observed. The bronchial epithelium revealed apoptotic changes. Also, there was a positive immune reaction for caspase-3 of the rat lung in alveolar cells and interalveolar septa. Exercise increased PO2 and SOD, attenuated the inflammatory edema [as assessed by alien nation of lung W/D ratio and the lung showed more or less normal architecture with few areas of slight thickening of the interalveolar sepia. The bronchioles showed normal epithelium and circularly arranged smooth muscles], accompanied by significant reduction in plasma levels of MDA, TNF-alpha and IL- 6 in E+ L/R group when compared with I/R group. A slight positive immune reaction for caspase-3 was shown
Conclusions: Exercise could attenuate the remote lung injury after hindlimb ischaemia reperfusion injury through its ant: oxidative, anti-inflammatory and anti apoptotic effects