Effects of therapeutic ultrasound on the endothelial function of patients with type 2 diabetes mellitus

Type 2 diabetes mellitus (T2DM) is characterized by endothelial dysfunction that causes micro- and macrovascular complications. Low intensity therapeutic ultrasound (LITUS) may improve endothelial function, but its effects have not been investigated in these patients. The aim of our study was to compare the effects of pulsed (PUT) and continuous (CUT) waveforms of LITUS on the endothelium-dependent vasodilation of T2DM patients. The present randomized crossover trial had a sample of twenty-three patients (7 men) diagnosed with T2DM, 55.6 (±9.1) years old, with a body mass index of 28.6 (±3.3) kg/m2. All patients were randomized and submitted to different waveforms (Placebo, CUT, and PUT) of LITUS and the arterial endothelial function was evaluated. The LITUS of 1 MHz was applied in pulsed (PUT: 20% duty cycle, 0.08 W/cm2 SATA), continuous (CUT: 0.4 W/cm2 SPTA), and Placebo (equipment off) types of waves during 5 min on the brachial artery. Endothelial function was evaluated using the flow-mediated dilation (FMD) technique. PUT (mean difference 2.08%, 95% confidence interval 0.65 to 3.51) and CUT (mean difference 2.32%, 95% confidence interval 0.89 to 3.74) increased the %FMD compared to Placebo. In the effect size analysis, PUT (d=0.65) and CUT (d=0.65) waveforms presented moderate effects in the %FMD compared to Placebo. The vasodilator effect was similar in the different types of waves. Pulsed and continuous waveforms of LITUS of 1 MHz improved the arterial endothelial function in T2DM patients.

Ad libitum consumption of milk supplemented with omega 3, 6, and 9 oils from infancy to middle age alters behavioral and oxidative outcomes in male mice

We tested the hypothesis that administration of omega (ω)-9, ω-3, and ω-6 to mice can prevent oxidative alterations responsible for behavioral and cognitive alterations related with aging. Twenty-eight-day-old mice received skim milk (SM group), SM enriched with omega oil mixture (EM group), or water (control group) for 10 and 14 months, equivalent to middle age. Mice were evaluated for behavioral alterations related to depression and memory and oxidative status [brain levels of thiobarbituric acid reactive substances (TBARS), reduced glutathione (GSH), and myeloperoxidase (MPO)]. The 10-month EM group increased immobility time during the forced swimming test compared with control, indicating increased stress response. The 14-month SM- and EM-treated groups increased sucrose consumption compared with control, showing an expanded motivational state. The 14-month SM group decreased the number of rearings compared with the 14-month control and EM groups. The number of entries and time spent in the central square of the open field was higher in the 10-month EM group than in the control, revealing an anxiolytic-like behavior. TBARS decreased in the hippocampus and striatum of the 10-month EM group compared with the control. A similar decrease was observed in the striatum of the 10-month SM group. GSH levels were higher in all 14-month treated groups compared with 10-month groups. MPO activity was higher in the 14-month EM group compared with the 14-month control and SM groups, revealing a possible pro-inflammatory status. In conclusion, omega oils induced conflicting alterations in middle-aged mice, contributing to enhanced behavior and anxiolytic and expanded motivational state, but also to increased stress response and pro-inflammatory alterations.

Pulmonary adenocarcinoma in a captive ocelot (Leopardus pardalis): morphologic and immunophenotypic characterization - case report / Adenocarcinoma pulmonar em uma jaguatirica (Leopardus pardalis) de cativeiro: caracterização morfológica e imunofenotípica - relato de caso

Pulmonary adenocarcinoma is a malignant epithelial neoplasia that usually arises from conducting airways or alveolar parenchyma. It has rarely been described in wild felids, with no previous reports in ocelots. In domestic cats it is a very aggressive neoplasm with a high metastatic rate that usually evolves to death. This report aimed to describe a pulmonary adenocarcinoma in a captive and senile ocelot (Leopardus pardalis), with a thorough morphologic and immunophenotypically characterization, evidencing the epithelial-mesenchymal transition (EMT) phenomenon in a high metastatic carcinoma, an important feature rarely described in veterinary medicine, even in domestic cats.(AU)

O adenocarcinoma pulmonar é uma neoplasia epitelial maligna originada do epitélio respiratório das vias aéreas inferiores e do parênquima alveolar. É uma neoplasia raramente descrita em felinos selvagens, sem nenhum relato em jaguatiricas. Em gatos domésticos, é uma neoplasia muito agressiva, com alta taxa de metástase, e geralmente evolui para o óbito do paciente. O presente relato objetiva descrever um adenocarcinoma pulmonar em uma jaguatirica (Leopardus pardalis) senil de cativeiro, com detalhada caracterização morfológica e imunofenotípica, evidenciando o fenômeno de transição epitelial-mesenquial (TEM) em um carcinoma altamente metastático, uma característica importante, com escassos relatos na medicina veterinária, mesmo em gatos domésticos.(AU)

Dioctophymosis in a free-ranging maned wolf (Chrysocyon brachyurus): clinical-therapeutic, ultrasonographic and pathological aspects - case report / [Dioctofimose em um lobo guará (Chrysocyon brachyurus) de vida livre: aspectos clínico-terapêuticos, ultrassonográficos e patológicos - relato de caso]

Dioctophymosis is the disease caused by Dioctophyma renale, a large nematode, popularly known as giant kidney worm, and whose definitive hosts are the domestic dog and many wild mammal species. There are some reports of maned wolf parasitism by D. renale, however, in most cases the reports are restricted to the finding of the parasite during necropsy, without clinical assessment. The present report aimed to describe the clinical-therapeutic, ultrasonographic and pathological aspects of D. renale parasitism in a free-ranging maned wolf, emphasizing the treatment with an association of doramectin, praziquantel, pyrantel pamoate, and febantel that resulted in complete elimination of the parasite.(AU)

A dioctofimose é a doença causada pelo Dioctophyma renale, um nemátodo grande, popularmente chamado de verme gigante do rim e que tem como hospedeiro definitivo o cão doméstico e inúmeras espécies de mamíferos silvestres. Existem alguns relatos do parasitismo do lobo-guará por D. renale, contudo, na maioria das vezes, os relatos se restringem apenas ao achado do parasita durante a necropsia, sem a correlação clínica. O presente relato objetiva descrever os aspectos clínico-terapêuticos, ultrassonográficos e patológicos do parasitismo por D. renale em um lobo-guará de vida livre, enfatizando o tratamento com uma associação de doramectina, praziquantel, pamoato de pirantel e febantel, o que resultou na eliminação completa do parasita.(AU)

Depression and anxiety disorders in patients with multiple sclerosis: association with neurodegeneration and neurofilaments

There is increasing evidence that neurofilament light chain (NF-L) can be considered as a biomarker for neuro-axonal damage. This polypeptide can be released into the cerebrospinal fluid (CSF) and the blood, where it can be quantified. The concentration of NF-L is elevated in patients with multiple sclerosis (MS) and psychiatric disorders. We aimed to investigate the NF-L levels in the CSF from treated MS patients and the relationship with depression or anxiety. The study involved three groups: control group (individuals without inflammation), the relapse-remitting multiple sclerosis (RRMS)-untreated group, and the RRMS-Fingo group (RRMS patients who were treated with fingolimod). MS disability was assessed by the Expanded Disability Status Scale, and depression and anxiety were evaluated by a neuropsychologist, using the Hospital Anxiety and Depression Scale, the Beck Depression Inventory-II, and the Beck Anxiety Inventory. Individual CSF samples were collected to measure NF-L levels. The results of the statistical analysis on levels of NF-L in the CSF of control subjects, RRMS-untreated patients, and RRMS-Fingo patients were significant. The relationship between depression and anxiety in RRMS-Fingo patients and NF-L levels was not statistically significant. In conclusion, MS events such as anxiety and depression appear to contribute to the onset of clinical relapses, subclinical cases, and neurodegeneration.

Biomass components and structure of massai grass fertilized with nitrogen and grazed by sheep / Componentes da biomassa e estrutura do capim-massai adubado com nitrogênio e pastejado por ovinos

To evaluate the biomass components of massai grass subjected to nitrogen (N) fertilization levels (control - 0; 400; 800 and 1200 kg ha-1 year-1 of N) and under rotational stocking with sheep, this study was undertaken using a completely randomized design with measurements repeated in time. A quadratic response was observed reaching maximum values as the levels of nitrogen fertilization increased for green forage biomass, green leaf biomass, green stem biomass (5,172.9; 4,146.3; 1,033.9 kg ha-1 cycle-1, respectively), forage total density (179.1 kg ha-1 cm-1), canopy height (36.8 cm) and live material/dead material ratio (4.0) at levels 896; 934; 797; 879.2; 751.4 and 1,161 kg ha-1 year-1 of N, respectively. For all variables, oscillation was observed between the grazing cycles studied. Nitrogen fertilization has a positive response on the biomass components and canopy structure of massai grass.(AU)

Objetivou-se avaliar os componentes da biomassa do capim-massai sob lotação rotativa com ovinos e adubado com doses de nitrogênio (controle - 0; 400; 800 e 1200 kg de N ha -1 ano -1 ), em delineamento inteiramente ao acaso, com medidas repetidas no tempo. Constatou-se resposta quadrática, alcançando valores máximos com o incremento das doses de nitrogênio para as produções de biomassa de forragem verde, de lâmina foliar verde, de colmo verde (5172,9; 4146,3; 1033,9 kg ha -1 ciclo -1 , respectivamente) e para a densidade total de forragem (179,1 kg ha -1 cm -1 ), altura do dossel (36,8 cm) e relação material vivo/material morto (4,0) nas doses 896; 934; 797; 879,2; 751,4 e 1161 kg ha -1 ano -1 de N, respectivamente. Para todas as variáveis, verificou-se oscilação entre os ciclos de pastejo estudados. A adubação nitrogenada exerce respostas positivas sobre os componentes da biomassa e a estrutura do dossel do capim-massai.(AU)

Análise bioeconômica do farelo de arroz na recria de bezerras de corte em azevém / Bioeconomics analyses of rice brain use for reaning of beef heifers on Italian ryegrass pasture

Objetivou-se avaliar a economicidade, o desempenho produtivo e o desenvolvimento das estruturas corporais relacionadas ao trato reprodutivo de bezerras de corte mantidas exclusivamente em pastagem de azevém (Lolium multiflorum Lam.) ou em pastagem de azevém recebendo 0,5 e 1,0% do peso corporal (PC) de farelo de arroz integral (FAI) como suplemento. O método de pastejo foi o rotativo, e o intervalo entre pastejos foi estabelecido considerando-se a soma térmica necessária para a emissão de 1,5 folha de azevém (187,5ºC). O delineamento experimental foi o inteiramente ao acaso, com medidas repetidas no tempo. O ganho médio diário das bezerras não diferiu entre os sistemas alimentares. A taxa de lotação e o ganho de peso por área foram maiores quando as bezerras receberam 1,0% do PC de farelo de arroz integral. A maior margem bruta foi observada quando as bezerras receberam 0,5% de FAI. O custo variável obtido para os diferentes sistemas alimentares apresentou comportamento crescente em função dos níveis de FAI, superando o uso exclusivo do azevém em 49,4% e 81,8%. O peso corporal final, o escore de condição corporal e o escore do trato reprodutivo não diferiram entre os sistemas alimentares. Considerando-se um animal adulto com peso corporal médio de 450kg, as bezerras ao início do experimento apresentaram 34% do peso corporal adulto e peso corporal final médio de 56% do peso adulto. A utilização de níveis de farelo de arroz integral (FAI) na recria de bezerras de corte sob pastejo rotativo em azevém não modifica o ganho médio diário e o escore do trato reprodutivo. O fornecimento de 1% de FAI mostrou efeito substitutivo, proporcionando um incremento de 31,2% na taxa de lotação e de 38,3% no ganho por área. O sistema alimentar AZ0,5 permitiu uma maior margem bruta; já o retorno financeiro direto foi positivo em todos os sistemas alimentares, com melhor retorno calculado para o uso exclusivo do azevém.(AU)

The experiment was carried out with the objective of evaluating the gross margin, the productive and reproductive performance of beef heifers kept exclusively on ryegrass pasture (Lolium multiflorum Lam.) or ryegrass receiving 0.5 and 1.0% of body weight (BW) of whole rice bran as supplement. The grazing method was the rotational and the interval between grazings was set by the thermal sum required for the appearing of 1.5 ryegrass leaf (187.5ºC). The experimental design was completely randomized with repeated measures. Heifers average daily gain did not differ between the feeding systems. The stocking rate and weight gain per area were higher when heifers received 1.0% BW of whole rice bran. The higher gross margin was observed when heifers received 0.5% BW. The variable cost obtained for the different feeding systems showed increasing behavior depending on the WRB levels, surpassing the exclusive use of ryegrass in 49.4% and 81.8%. The final body weight, body condition score and reproductive tract score did not differ between the feeding systems. Considering an adult animal with an average body weight of 450kg, the heifers at the beginning of the experiment showed 34% of mature body weight and final average body weight of 56% of adult weight. The use of rice bran levels (FAZ) in the rearing of beef heifers under rotational grazing on ryegrass does not change the average daily gain and the reproductive tract score. The supply of 1% FAI showed substitutive effect, providing an increase in capacity of 31,2% and 38.3% rate of the gain per area. The food system AZ0,5 allowed a higher gross margin, as the economic return was positive in all food systems, with better return calculated for the exclusive use of ryegrass.(AU)

Forage intake and performance of heifers fed rice bran on Alexandergrass pasture / Ingestão de forragem e performance de bezerros alimentados com farelo de arroz integral em pasto de papuã

he study was carried out to evaluate the forage intake and performance of beef heifers from 15 to 18 months of age, grazing on Alexandergrass (Urochloaplantaginea (Link) Hitch) fed rice bran (RB; 0, 0.5 and 1.0% body weight (BW)). The experiment consisted of a completely randomized design with repeated measurement arrangement. Rice bran increased the total dry matter (DM) intake, total crude protein intake and total digestible nutrients intake. Heifers that received 1.0% BW of RB reduced forage DM intake, increasing by 18% stocking rate (SR) and by 27% BW gain per area in comparison with the system exclusively on pasture. In the system in which the heifers were fed 0.5% BW of RB, SR and gain per area were similar to other systems. In this feeding system, we observed average daily gain 18% higher than when heifers were exclusively on pasture and gain similar to that observed when using 1.0% BW of RB. In Alexandergrass pasture it is recommended to supply 0.5% BW of RB for beef heifers from 15 to 18 months of age, to promote better productive responses compared to heifers exclusively on pasture and similar to those observed when supplying 1.0% BW of RB.(AU)

O presente trabalho foi desenvolvido com o objetivo de avaliar o consumo de forragem e o desempenho de novilhas de corte dos 15 aos 18 meses de idade, em pastejo em papuã (Urochloaplantaginea(Link) Hitch), recebendo farelo de arroz integral (FAI) como suplemento (0, 0,5 e 1,0% do peso corporal (PC)). O delineamento experimental foi o inteiramente ao acaso, com medidas repetidas no tempo. O uso de FAI aumentou o consumo total de matéria seca (MS), o consumo total de proteína bruta e de nutrientes digestíveis totais em relação às novilhas exclusivamente em pastejo. As novilhas que receberam 1,0% do PC de FAI reduziram o consumo de MS da forragem, aumentando em 18% a taxa de lotação (TxLot) e em 27% o ganho de PC por hectare (GPA) em comparação com o sistema exclusivamente a pasto. No sistema em que as novilhas receberam 0,5% do PC de FAI, a TxLot e o GPA foram similares aos demais sistemas. Nesse sistema alimentar, foi observado GDM 18% superior em relação às novilhas exclusivamente em pastejo e ganho similar ao uso de 1,0% do PC de FAI. Em pastagem de papuã, é recomendado o uso de 0,5% do PC de FAI para novilhas de corte dos 15 aos 18 meses de idade, por promover respostas produtivas superiores em relação às novilhas exclusivamente em pastejo e semelhantes às observadas quando fornecido 1,0% do PC de FAI.(AU)

Padrões de deslocamento de bezerras de corte recebendo suplemento em pastagem de azevém / Displacement patterns of beef heifers receiving supplement in Italian ryegrass pasture

O objetivo deste trabalho foi avaliar o tempo de permanência, o número de bocados nas estações e o deslocamento entre estações alimentares por bezerras de corte em pastagem de azevém (Lolium multiflorum Lam.) e recebendo farelo de arroz integral como suplemento em três níveis (0,0; 0,5 e 1,0% PC). O método de pastejo foi rotacionado, com número variável de animais. O período de descanso foi estabelecido pela soma térmica (ST) de 187,5ºC, equivalente à ST necessária para a emissão de 1,5 folhas de azevém. O delineamento experimental foi inteiramente ao acaso, com medidas repetidas no tempo, três níveis de suplemento e seis ciclos de pastejo. O tempo de permanência em cada estação alimentar (9,4s) foi semelhante para bezerras exclusivamente a pasto e as que receberam 1,0% PC de suplemento. As bezerras que receberam 0,5% PC de suplemento permanecem 1,1s a mais na estação alimentar quando comparadas com as bezerras nos demais níveis. O deslocamento entre estações alimentares (1,7 passos) e o número de bocados em cada estação alimentar (sete bocados) foram semelhantes independentemente do nível de farelo de arroz fornecido. As bezerras, ao receberem 0,5% PC de farelo de arroz, são mais dependentes da presença de lâminas foliares no estrato pastejável do dossel.(AU)

The aim of this study was to evaluate the length of stay and number of bites in the feeding stations and displacement between feeding stations for beef heifers on ryegrass pasture (Lolium multiflorum Lam.) and receiving rice bran as a supplement in three levels (0.0; 0.5 and 1.0% BW). The grazing method was rotational with variable number of animals. The interval between grazing was set by the thermal sum (TS) of 187.5ºC equivalent to TS necessary for externalization of 1.5 leaf lamina of ryegrass. The experimental design was completely randomized with repeated measures on time, three levels of supplement and six grazing periods. The stay time at each feeding station (9.4 sec.) was similar for heifers exclusively on pasture and receiving 1.0% supplement BW. Heifers that received 0.5% supplement BW remained 1.1 sec. longer at the feeding station than heifers on other supplement levels. Displacement between feeding stations (1.7 steps) and the number of bites in each feeding station (7 bites) were similar regardless of the supplied rice bran level. Heifers receiving 0.5% rice bran BW are more dependent on the presence of leaf blades in grazeable stratum of canopy.(AU)

Estudio Comparativo entre la Técnica de Anestesia Local Controlada por Computador y la Técnica de Anestesia Local Convencional / Comparative Study Between the Conventional Local Anesthetic Technique and the Computer-controlled Local Anesthetic

El control del dolor es uno de los aspectos más importantes para propiciar al paciente un comportamiento positivo en la consulta odontológica. Sin embargo, esto aún constituye un desafío para el cirujano dentista cuando trata pacientes ansiosos, ya que la administración de la anestesia local es lo que determina que muchos pacientes eviten el tratamiento odontológico. Con el propósito de disminuir la sensación dolorosa en pacientes y promover mayor comodidad, los profesionales han desarrollado un método de anestesia computarizada. El objetivo de esta investigación fue comparar la técnica de anestesia local convencional con la técnica de anestesia local controlada por computador. Participaron 30 pacientes voluntarios adultos que tenían indicación para exodoncia de terceros molares inferiores de ambos lados. Los pacientes, elegidos de forma aleatoria, fueron sometidos a las dos técnicas anestésicas mencionadas (convencional y controlada por computador). Aunque el grado de satisfacción de los profesionales con la técnica anestésica controlada por computador fue significativa, la mayoría declaró preferir el método convencional. No hubo cambios hemodinámicos significativos en cualquiera de los dos métodos utilizados en este estudio. La técnica anestésica controlada por computador se mostró sencilla, eficaz y segura. Los pacientes que participaron en este estudio respondieron mejor al método de anestesia controlada por computador, reportando menos dolor.

Pain control is one of the most important aspects contributing that the patient has a positive behavior in the dental office. However, this still constitutes a challenge for the dentist when treating anxious patients, because the administration of local anesthesia is the cause of many patients to avoid dental treatment. With the purpose of reducing pain sensation for patients and promote greater comfort for patients and professionals was developed a computerized anesthesia injection method. The aim of this investigation was to compare the conventional local anesthetic technique with the computer-controlled local anesthetic. Participated in this study 30 volunteers patients who had indication for bilateral surgical extraction of lower third molars. The patients, randomly selected, were subjected to each of the two mentioned anesthetic techniques (conventional and computer-controlled). Although the degree of professional satisfaction with the computer-controlled local anesthetic technique has been significant, the most of them reported to prefer the conventional method. There was no significant hemodynamic changes in any of the two methods used in this study. The computer-controlled local anesthetic technique was simple, effective and safe. The patients enrolled in this study responded better to the computer-controlled local anesthetic technique, reporting less pain.

Palatoglossus muscle neuroanatomy: a review

The palatoglossus muscle is classically described as an extrinsic muscle of the tongue. However, this descriptionis not consensus among the researchers, is one that sometimes it is not considered a muscle of the tongue.Thus, the objective of this study is to discuss some neuroanatomical aspects of palatoglossus muscle that mayhelp explain this aspect. Furthermore, this study shall be useful for clinicians, surgeons and academics thatmanipulate and keep particular interest for this anatomical site.

Levantamento etnobotânico de plantas medicinais utilizadas pelos moradores do povoado de Manejo, Lima Duarte - MG / Ethnobotanical survey of the medicinal plants used by dwellers of Manejo Village, Lima Duarte-Minas Gerais State, Brazil

O objetivo deste estudo foi o levantamento etnobotânico de plantas medicinais utilizadas pela população do povoado de Manejo, Lima Duarte (MG). O estudo foi realizado mediante visitas às casas dos moradores que responderam a questionário relacionado às espécies de plantas que são cultivadas, respectivas partes utilizadas, bem como as formas de preparo e quais doenças são tratadas com as plantas. Foram realizadas 41 visitas resultando em citações de 100 diferentes espécies medicinais, sendo a maioria cultivada nos quintais e outras nativas da região, as quais também são utilizadas pelos moradores. Exemplares foram coletados e depositados no herbário CESJ, da Universidade Federal de Juiz de Fora. As espécies mais citadas foram Mentha sp., Lippia alba (Mill.) N. E. Brown, Foeniculum vulgare Mill., Achyrocline satureioides (Lam.) DC., Bidens pilosa L., Mentha pulegium L., Mikania glomerata Spreng., Rosa sp. e Plantago major L. As doenças mais tratadas com as plantas medicinais em Manejo são gripes e resfriados, problemas estomacais, cólicas menstruais e infecções no útero, verminose, problemas renais, ansiedade e estresse. As partes mais utilizadas são as folhas, e a forma de preparo mais comum das plantas são os chás por infusão. A construção de horta comunitária no povoado pode valorizar o emprego das plantas medicinais, sobretudo pelos mais jovens, mantendo a tradição do uso pelas futuras gerações.

The aim of this study was the ethnobotanical survey of medicinal plants used by the population of Manejo Village, Lima Duarte (Minas Gerais State), Brazil. The study was performed by means of visitations to the houses of dwellers who answered a questionnaire related to the plant species that are cultivated, respective parts that are used, as well as preparation forms and which diseases are treated with the plants. A total of 41 visitations were done, resulting in citation of 100 different medicinal species, most of which were cultivated in backyards while others were native to that region, which were also used by the dwellers. Specimens were collected and deposited in CESJ herbarium, of the Federal University of Juiz de Fora. The most cited species were Mentha sp., Lippia alba (Mill.) N. E. Brown, Foeniculum vulgare Mill., Achyrocline satureioides (Lam.) DC., Bidens pilosa L., Mentha pulegium L., Mikania glomerata Spreng., Rosa sp. and Plantago major L. The diseases most treated with medicinal plants in Manejo Village are flu and cold, stomach disorders, menstrual cramps and uterus infections, helminth infections, kidney problems, anxiety and stress. The most used parts are leaves, and the most common form of plant preparation is infusion tea. The construction of a community garden in the village may value the use of medicinal plants, especially by the young, maintaining the tradition of their use by future generations.

Ossification of styloid ligament and its clinical implication a report of human casses

A case of ossification of the right styloid ligament in an adult human caucasian skull was verified duringroutine activities of the Laboratory of Human Anatomy at the University of Santa Cruz do Sul - Brazil. Thisstudy presents its clinical considerations and applications. The presence of other anatomical variations was notverified.

Molecular detection of Leishmania infantum in naturally infected Phlebotomus perniciosus from Algarve Region, Portugal.

Background & objectives: In Portugal, Phlebotomus perniciosus and P. ariasi, (Subgenus Larroussius; Diptera: Psychodidae) are the proven vectors of leishmaniasis caused by Leishmania infantum. The Algarve Region in southern Portugal has been considered an endemic focus of leishmaniasis since 1980s. The main objective of the present study was to validate a molecular approach to detect Leishmania infection in phlebotomines based on DNA extraction from the female sandfly whole body, minus genitalia, followed by PCR for application on epidemiological surveys. Methods: In Algarve Region, from early May until early November 2006, sandflies were captured by CDC miniature light-traps. kDNA-PCR and ITS1-PCR were used to screen the presence of Leishmania DNA in female sandflies after species identification by entomological keys. Results: A total of 474 sandflies were collected in 108 biotopes. One female of P. perniciosus, the predominant species, was found infected with L. infantum reflecting an overall infection rate of 0.47%. Interpretation & conclusion: PCR associated with morphological characterization of the sandflies will be a powerful epidemiological tool for the determination of the number of phlebotomines infected with Leishmania spp in nature. In addition, the simultaneous occurrence of dogs and P. perniciosus infected with L. infantum shows that Algarve continues to be an endemic focus of canine leishmaniasis. Furthermore, as P. sergenti and P. papatasi which transmit L. tropica and L. major, respectively were present, the future introduction of these two Leishmania species in southern region of Portugal should not be neglected.

Clinical utility of standard base excess in the diagnosis and interpretation of metabolic acidosis in critically ill patients

The aims of this study were to determine whether standard base excess (SBE) is a useful diagnostic tool for metabolic acidosis, whether metabolic acidosis is clinically relevant in daily evaluation of critically ill patients, and to identify the most robust acid-base determinants of SBE. Thirty-one critically ill patients were enrolled. Arterial blood samples were drawn at admission and 24 h later. SBE, as calculated by Van Slyke's (SBE VS) or Wooten's (SBE W) equations, accurately diagnosed metabolic acidosis (AUC = 0.867, 95 percentCI = 0.690-1.043 and AUC = 0.817, 95 percentCI = 0.634-0.999, respectively). SBE VS was weakly correlated with total SOFA (r = -0.454, P < 0.001) and was similar to SBE W (r = -0.482, P < 0.001). All acid-base variables were categorized as SBE VS <-2 mEq/L or SBE VS <-5 mEq/L. SBE VS <-2 mEq/L was better able to identify strong ion gap acidosis than SBE VS <-5 mEq/L; there were no significant differences regarding other variables. To demonstrate unmeasured anions, anion gap (AG) corrected for albumin (AG A) was superior to AG corrected for albumin and phosphate (AG A+P) when strong ion gap was used as the standard method. Mathematical modeling showed that albumin level, apparent strong ion difference, AG A, and lactate concentration explained SBE VS variations with an R² = 0.954. SBE VS with a cut-off value of <-2 mEq/L was the best tool to diagnose clinically relevant metabolic acidosis. To analyze the components of SBE VS shifts at the bedside, AG A, apparent strong ion difference, albumin level, and lactate concentration are easily measurable variables that best represent the partitioning of acid-base derangements.

p53 immunostaining is correlated with reduced survival and is not correlated with gene mutations in resected pulmonary large cell carcinomas

Malignancy of pulmonary large cell carcinomas (LCC) increases from classic LCC through LCC with neuroendocrine morphology (LCCNM) to large cell neuroendocrine carcinomas (LCNEC). However, the histological classification has sometimes proved to be difficult. Because the malignancy of LCC is highly dependent on proteins with functions in the cell cycle, DNA repair, and apoptosis, p53 has been targeted as a potentially useful biological marker. p53 mutations in lung cancers have been shown to result in expression and protein expression also occurs in the absence of mutations. To validate the importance of both p53 protein expression (by immunostaining) and p53 gene mutations in lung LCC (by PCR-single strand conformational polymorphism analysis of exons 5, 6, 7, and 8) and to study their relationships with clinical factors and sub-classification we investigated the correlation of p53 abnormalities in 15 patients with LCC (5 classic LCC, 5 LCNEC, and 5 LCCNM) who had undergone resection with curative intent. Of these patients, 5/15 expressed p53 and none had mutant p53 sequences. There was a negative survival correlation with positive p53 immunostaining (P = 0.05). After adjustment for stage, age, gender, chemotherapy, radiotherapy, and histological subtypes by multivariate analysis, p53 expression had an independent impact on survival. The present study indicates that p53 assessment may provide an objective marker for the prognosis of LCC irrespective of morphological variants and suggests that p53 expression is important for outcome prediction in patients with the early stages of LCC. The results reported here should be considered to be initial results because tumors from only 15 patients were studied: 5 each from LCC, LCNEC and LCCNM. This was due to the rarity of these specific diseases.

The Candida albicans AAA ATPase homologue of Saccharomyces cerevisiae Rix7p (YLL034c) is essential for proper morphology, biofilm formation and activity of secreted aspartyl proteinases

Proper morphology is essential for the ability of Candida albicans to switch between yeast and hyphae and thereby sustain its virulence. Here we identified, by differential screening, a novel C. albicans AAA ATPase encoding gene, CaYLL34 (RIX7), with enhanced expression in hyphae. Phylogenetic analysis suggests that CaYLL34 belongs to a [quot ]VCP-like[quot ] subgroup of AAA ATPases essential for yeast viability and contains a bipartite nuclear localization signal. Inactivation of one copy of CaYLL34, by the URA-Blaster method, generated the heterozygous mutant strain M61. This strain has severe phenotypic alterations, such as a highly increased vacuole, abnormal cell shape and reduced growth in different conditions. Also, major pathogenicity factors are affected in M61, for instance, a significant decrease of hypha formation (>90%), surface biofilm adhesion (86%) and secreted aspartyl proteinase activity (76.5%). Our results show that the partial impairment of CaYll34p cellular levels is sufficient to affect the proper cellular morphology and pathogenicity factors and suggest that this protein is required for biogenesis of ribosomal subunits. Accordingly, we propose that the product of CaYLL34 could be tested as a novel target for antifungal drugs.

Atividade antimicrobiana de bactérias ácido-lácticas isoladas de queijos de coalho artesanal e industrial frente a microrganismos indicadores / Antimicrobial activity of lactic acid bacteria isolated from artisanal and industrial

Quatro cepas de Lactobacillus spp. e duas cepas de Lactococcus spp. isoladas de queijos de coalho artesanal e industrial foram testadas quanto às suas atividades antimicrobianas. Observou-se atividade antagonista dessas bactérias ácido-lácticas frente a elas, a outras bactérias ácido-lácticas isoladas de queijo de coalho, aos patógenos isolados dos mesmos queijos e a cepas de patógenos de referência. Verificou-se diferença (P<0,05) entre as atividades antagonistas, exceto quando as próprias bactérias ácido-lácticas foram utilizadas como reveladoras. Lactobacillus spp. apresentaram as atividades antagonistas mais potentes.

Padronização invasiva da pressão arterial auricular caudal em cães submetidos à anestesia inalatória

O artigo não apresenta resumo.