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1.
The Korean Journal of Parasitology ; : 387-392, 2020.
Article | WPRIM | ID: wpr-833778

Résumé

A serological survey was performed using ELISA to estimate the prevalence of tissue and luminal helminthic infections among hospital patients in Hanoi region, Vietnam. An overall seroprevalence of tissue and luminal helminthiases was 64.0% (95% CI 61.2-66.8) among 1,120 patients who visited Hanoi Medical University Hospital, Vietnam in 2018. The highest seroprevalence was observed against Toxocara spp. (59.0%), followed by Strongyloides stercoralis (46.3%), Gnathostoma spp. (25.5%), cysticercus (12.8%), Angiostrongylus cantonensis (10.5%), Fasciola spp. (11.1%), and Clonorchis sinensis (8.7%). Mono-infection by one species (11.1%) was lower than multiple infections (53.0%) (P0.05). Children (<15 years) revealed lower seroprevalence (34.0%) than adults (68.4%), and the age group 51-70 years revealed the highest seroprevalence (76.0%). Among the seropositive patients, eosinophilia (≥8.0%) was noted in 80.2%. The present results suggested active transmission of various tissue and luminal helminths among people in Hanoi, Vietnam.

2.
The Korean Journal of Parasitology ; : 451-456, 2020.
Article | WPRIM | ID: wpr-833772

Résumé

This is a report of 2 cases of human hydatidosis caused by Echinococcus ortleppi in Vietnam. The patients were a 12-year-old male (case 1) having a cyst of 10.0×9.0 cm size in the lung and a 50-year-old female with a 3.0×3.3 cm-sized cyst in the heart. Eosinophilia was 33.7% in the male and 45.8% in the female patient. C-reactive protein was increased to 16.5 mg/L in the male and 18.2 mg/L in the female. Both patients were positive for ELISA at OD=2.5 and 3.1, respectively. Echinococcus protoscolices were collected from the cysts by amniocentesis and surgery. The protoscolices were identified as E. ortleppi by morphology and analysis of mitochondrial NADH dehydrogenase 1 (nad1) gene sequence. Both patients were cured by surgical resection of the hydatid cyst combined with albendazole medication. The E. ortleppi infection in lung is the second report, and the other in the heart is the first in Vietnam.

3.
The Korean Journal of Parasitology ; : 453-461, 2018.
Article Dans Anglais | WPRIM | ID: wpr-742278

Résumé

The aim of this study is to delineate ‘admixed hybrid’ and ‘introgressive’ Fasciola genotypes present in the Fasciola population in Vietnam. Adult liver flukes collected from ruminants in 18 Provinces were morphologically sorted out by naked eyes for small (S), medium (M) and large (L) body shapes; and human samples (n=14) from patients. Nuclear ribosomal (rDNA) ITS1 and ITS2, and mitochondrial (mtDNA) nad1 markers were used for determination of their genetic status. Total 4,725 worm samples of ruminants were tentatively classified by their size: 6% (n=284) small (S)-, 13% (n=614) medium (M)-, and 81% (n=3,827) large (L)-forms. All the representative (n=120, as 40 each group) and 14 human specimens, possessed maternal mtDNA of only F. gigantica and none of F. hepatica. Paternally, all (100%) of the L-(n=40) and 77.5% (n=31) of the M-flukes had single F. gigantica rDNA indicating ‘pure’ F. gigantica. A majority (90%, n=36) of the S- and 15% (n=6) of the M-worms had single F. hepatica rDNA, indicating their introgressive; the rest (10%, n=4) of the S- and 7.5% (n=3) of the M-flukes had mixture of both F. gigantica and F. hepatica rDNAs, confirming their admixed hybrid genetic status. Fourteen human samples revealed 9 (64%) of pure F. gigantica, 3 (22%) of introgressive and 2 (14%) of admixed hybrid Fasciola spp. By the present study, it was confirmed that the small worms, which are morphologically identical with F. hepatica, are admixed and/or introgressive hybrids of Fasciola spp., and able to be the pathogens of human fascioliasis.


Sujets)
Adulte , Humains , ADN mitochondrial , ADN ribosomique , Fasciola hepatica , Fasciola , Fasciolase , Génotype , Foie , Ranonculaceae , Ruminants , Vietnam
4.
The Korean Journal of Parasitology ; : 471-476, 2016.
Article Dans Anglais | WPRIM | ID: wpr-99319

Résumé

To know the infection status of helminths in primary schoolchildren of southern parts of Vietnam, we performed an epidemiological study in Krong Pac district, Dak Lak Province, Vietnam. A total of 1,206 stool specimens were collected from ethnic Ede schoolchildren in 4 primary schools in 2015 and examined by the Kato-Katz technique. In addition, stool cultures were done by the Harada-Mori method to obtain hookworm larvae and then to clarify the species of hookworms infected. The results showed that the helminth infection rate was 25.0%, including 2.0% Ascaris lumbricoides, 0.33% Trichuris trichiura, and 22.8% hookworm infections. The average intensity of infection was 102.0 eggs per gram of feces (EPG) for Ascaris, 36.0 EPG for Trichuris, and 218.0 EPG for hookworms. ITS1 gene sequences of the hookworm larvae were identical with those of Necator americanus (100% homology) reported in GenBank. It has been confirmed in this study that the hookworm, N. americanus, is a dominant helminth species infected in primary schoolchildren of a southern part of Vietnam. Public health attention is needed for control of hookworm infections among schoolchildren in surveyed areas of Vietnam.


Sujets)
Humains , Ancylostomatoidea , Ascaris , Ascaris lombricoides , Bases de données d'acides nucléiques , Oeufs , Études épidémiologiques , Fèces , Helminthes , Infections à ankylostomes , Larve , Méthodes , Necator americanus , Ovule , Prévalence , Santé publique , Trichuris , Vietnam
5.
The Korean Journal of Parasitology ; : 713-717, 2015.
Article Dans Anglais | WPRIM | ID: wpr-91233

Résumé

A 23-year-old female residing in a village of Cao Bang Province, North Vietnam, visited the Hospital of Hanoi Medical University in July 2013. She felt dim eyes and a bulge-sticking pain in her left eye for some days before visiting the hospital. In the hospital, a clinical examination, an eye endoscopy, and an operation were carried out. A nematode specimen was collected from the eye of this patient. The body of this worm was thin and long and measured 22.0x0.3 mm. It was morphologically suggested as an immature female worm of Angiostrongylus cantonensis. By a molecular method using 18S rRNA gene, this nematode was confirmed as A. cantonensis. This is the first molecular study for identification of A. cantonensis in Vietnam.


Sujets)
Animaux , Femelle , Humains , Jeune adulte , Angiostrongylus cantonensis/classification , Séquence nucléotidique , Oeil/parasitologie , Maladies de l'oeil/diagnostic , Données de séquences moléculaires , Infections à Strongylida/diagnostic , Vietnam
6.
The Korean Journal of Parasitology ; : 125-129, 2014.
Article Dans Anglais | WPRIM | ID: wpr-20009

Résumé

Several reports on taeniasis and cysticercosis in Vietnam show that they are distributed in over 50 of 63 provinces. In some endemic areas, the prevalence of taeniasis was 0.2-12.0% and that of cysticercosis was 1.0-7.2%. The major symptoms of taeniasis included fidgeted anus, proglottids moving out of the anus, and proglottids in the feces. Clinical manifestations of cysticercosis in humans included subcutaneous nodules, epileptic seizures, severe headach, impaired vision, and memory loss. The species identification of Taenia in Vietnam included Taenia asiatica, Taenia saginata, and Taenia solium based on combined morphology and molecular methods. Only T. solium caused cysticercosis in humans. Praziquantel was chosen for treatment of taeniasis and albendazole for treatment of cysticercosis. The infection rate of cysticercus cellulosae in pigs was 0.04% at Hanoi slaughterhouses, 0.03-0.31% at provincial slaughterhouses in the north, and 0.9% in provincial slaughterhouses in the southern region of Vietnam. The infection rate of cysticercus bovis in cattle was 0.03-2.17% at Hanoi slaughterhouses. Risk factors investigated with regard to transmission of Taenia suggested that consumption of raw meat (eating raw meat 4.5-74.3%), inadequate or absent meat inspection and control, poor sanitation in some endemic areas, and use of untreated human waste as a fertilizer for crops may play important roles in Vietnam, although this remains to be validated.


Sujets)
Animaux , Humains , Albendazole/usage thérapeutique , Anthelminthiques/usage thérapeutique , Antiprotozoaires/usage thérapeutique , Bovins/parasitologie , Maladies des bovins/parasitologie , Cysticercose/traitement médicamenteux , Fèces/parasitologie , Viande/parasitologie , Praziquantel/usage thérapeutique , Aliments crus/effets indésirables , Facteurs de risque , Suidae/parasitologie , Maladies des porcs/parasitologie , Vietnam/épidémiologie
7.
The Korean Journal of Parasitology ; : 563-567, 2013.
Article Dans Anglais | WPRIM | ID: wpr-155355

Résumé

An ocular Toxocara canis infection is reported for the first time in Vietnam. A 34-year-old man residing in a village of Son La Province, North Vietnam, visited the National Eye Hospital (NEH) in August 2011. He felt a bulge-sticking pain in his left eye and loss of vision occurred over 3 months before visiting the hospital. The eye examination in the hospital showed damage of the left eye, red eye, retinal fibrosis, retinal detachment, inflammation of the eye tissues, retinal granulomas, and a parasitic cyst inside. A larva of Toxocara was collected with the cyst by a medical doctor by surgery. Comparison of 264 nucleotides of internal transcribed spacer 2 (ITS2) of ribosomal DNA was done between our Vietnamese Toxocara canis and other Toxocara geographical isolates, including Chinese T. canis, Japanese T. canis, Sri Lankan T. canis, and Iranian T. canis. The nucleotide homology was 97-99%, when our T. canis was compared with geographical isolates. Identification of a T. canis infection in the eye by a molecular method was performed for the first time in Vietnam.


Sujets)
Adulte , Animaux , Humains , Mâle , Séquence nucléotidique , ADN des helminthes/composition chimique , ADN ribosomique/composition chimique , Espaceur de l'ADN ribosomique/composition chimique , Parasitoses oculaires/diagnostic , Larve , Données de séquences moléculaires , Phylogenèse , Alignement de séquences , Analyse de séquence d'ADN , Toxocara canis/classification , Toxocarose/diagnostic , Vietnam
8.
The Korean Journal of Parasitology ; : 221-223, 2012.
Article Dans Anglais | WPRIM | ID: wpr-175372

Résumé

A 26-year-old man residing in a village of Thai Nguyen Province, North Vietnam, visited the Thai Nguyen Provincial Hospital in July 2008. He felt a bulge-sticking pain in his left eye and extracted 5 small nematode worms by himself half a day before visiting the hospital. Two more worms were extracted from his left eye by a medical doctor, and they were morphologically observed and genetically analyzed on the mitochondrial cytochrome c oxidase 1 gene. The worms were 1 male and 1 female, and genetically identical with those of Thelazia callipaeda. By the present study, the presence of human T. callipaeda infection is first reported in Vietnam.


Sujets)
Adulte , Animaux , Humains , Mâle , Analyse de regroupements , Complexe IV de la chaîne respiratoire/génétique , Maladies de l'oeil/parasitologie , Microscopie , Phylogenèse , Infections à Spirurida/diagnostic , Thelazioidea/anatomie et histologie , Vietnam
9.
The Korean Journal of Parasitology ; : 317-325, 2012.
Article Dans Anglais | WPRIM | ID: wpr-45625

Résumé

The prevalence of foodborne trematode (FBT) metacercariae was investigated in fish from 2 localities of northern Vietnam in 2004-2005. Freshwater fish (9 species) were collected from local markets in Hanoi City (n=76) and Nam Dinh Province (n=79), and were examined for FBT metacercariae using the artificial digestion technique. Adult flukes were obtained from hamsters experimentally infected with the metacercariae at day 8 post-infection. Three (Haplorchis pumilio, Centrocestus formosanus, and Procerovum varium) and 6 (Haplorchis taichui, H. pumilio, C. formosanus, P. varium, Stellantchasmus falcatus, and Heterophyopsis continua) species of FBT metacercariae were detected in the 2 regions, respectively. Overall, among the positive fish species, H. pumilio metacercariae were detected in 104 (80.0%) of 130 fish examined (metacercarial density per infected fish; 64.2). C. formosanus metacercariae were found in 37 (40.2%) of 92 fish (metacercarial density; 14.7). P. varium metacercariae were detected in 19 (63.3%) of 30 fish (Anabas testudineus and Mugil cephalus) (metacercarial density; 247.7). S. falcatus metacercariae were found in all 10 M. cephalus examined (metacercarial density; 84.4). H. continua metacercariae (2 in number) were detected in 1 fish of Coilia lindmani. Morphologic characteristics of the FBT metacercariae and their experimentally obtained adults were described. The results have demonstrated that various FBT species are prevalent in northen parts of Vietnam.


Sujets)
Animaux , Cricetinae , Humains , Maladies des poissons/épidémiologie , Poissons , Maladies d'origine alimentaire/parasitologie , Heterophyidae/cytologie , Metacercariae/cytologie , Prévalence , Infections à trématodes/épidémiologie , Vietnam/épidémiologie
10.
The Korean Journal of Parasitology ; : 339-343, 2012.
Article Dans Anglais | WPRIM | ID: wpr-45622

Résumé

The 5th outbreak of trichinosis occurred in a mountainous area of North Vietnam in 2012, involving 24 patients among 27 people who consumed raw pork together. Six of these patients visited several hospitals in Hanoi for treatment. Similar clinical symptoms appeared in these patients within 5-8 days after eating infected raw pork, which consisted of fever, muscle pain, difficult moving, edema, difficult swallowing, and difficult breathing. ELISA revealed all (6/6) positive reactions against Trichinella spiralis antigen and all cases showed positive biopsy results for Trichinella sp. larvae in the muscle. The larvae detected in the patients were identified as T. spiralis (Vietnamese strain) by the molecular analysis of the mitochondrial cytochrome c oxidase subunit III (cox3) gene.


Sujets)
Adulte , Animaux , Femelle , Humains , Mâle , Antigènes d'helminthe/analyse , Épidémies de maladies , Complexe IV de la chaîne respiratoire/génétique , Test ELISA , Larve , Viande/parasitologie , Mitochondries/génétique , Muscles/parasitologie , Suidae , Trichinella spiralis/génétique , Trichinellose/épidémiologie , Vietnam/épidémiologie
11.
The Korean Journal of Parasitology ; : 137-141, 2012.
Article Dans Anglais | WPRIM | ID: wpr-146181

Résumé

From 2006 to 2010, hospitals in Hanoi treated 10 human patients for dirofilariasis. The worms were collected from parasitic places, and identification of the species was completed by morphology and molecular methods. Ten parasites were recovered either from the conjunctiva (n=9) or subcutaneous tissue (n=1). The parasites were 4.0-12.5 cm in length and 0.5-0.6 mm in width. Morphological observations suggested all parasites as Dirofilaria repens. Three of the 10 parasites (1 from subcutaneous tissue and 2 from eyes) were used for molecular confirmation of the species identification. A portion of the mitochondrial cox1 (461 bp) was amplified and sequenced. Nucleotide and amino acid homologies were 95% and 99-100%, respectively, when compared with D. repens (Italian origin, GenBank AJ271614; DQ358814). This is the first report of eye dirofilariasis and the second report of subcutaneous tissue dirofilariasis due to D. repens in Vietnam.


Sujets)
Adulte , Sujet âgé , Animaux , Femelle , Humains , Mâle , Adulte d'âge moyen , Cyclooxygenase 1/génétique , Dirofilaria repens/anatomie et histologie , Dirofilariose/diagnostic , Oeil/parasitologie , Maladies de l'oeil/parasitologie , Techniques de diagnostic moléculaire/méthodes , Parasitologie/méthodes , Phylogenèse , Réaction de polymérisation en chaîne , Analyse de séquence d'ADN , Similitude de séquences , Maladies de la peau/parasitologie , Tissu sous-cutané/parasitologie , Vietnam
12.
The Korean Journal of Parasitology ; : 449-455, 2011.
Article Dans Anglais | WPRIM | ID: wpr-107274

Résumé

We detected metacercariae of Echinostoma revolutum in Filopaludina sp. snails purchased from a local market in Nam Dinh Province for the first time in Vietnam. Adult flukes were harvested from experimentally infected hamsters at days 14 and 17 post-infection. The metacercariae were round, 170-190 microm (n=15) in diameter, with a cyst wall thickness of about 12 microm. A total of 37 collar spines were arranged around the head collar, and large excretory granules were seen in 2 canals of the excretory bladder. The 14-day old adult flukes were elongated, ventrally curved, and 5.0-7.2x0.8-1.3 mm (n=20). The head collar had a total of 37 collar spines arranged in 2 alternating rows, including 5 corner spines on each side. The cirrus sac contained a saccular seminal vesicle, a prostatic gland, and an unarmed cirrus. Two tandem testes were smooth or slightly lobed. Eggs were ovoid to elliptical, 110-118x70-75 microm. These morphological characters were similar to those of E. revolutum and E. jurini. We tentatively identified it as E. revolutum because the validity of E. jurini remains to be elucidated. The taxonomic relationship of E. revolutum and E. jurini is discussed.


Sujets)
Animaux , Cricetinae , Femelle , Echinostoma/anatomie et histologie , Infection à Echinostoma/parasitologie , Metacercariae/anatomie et histologie , Escargots/parasitologie , Vietnam
13.
Journal of Practical Medicine ; : 6-8, 2005.
Article Dans Vietnamien | WPRIM | ID: wpr-4065

Résumé

Toan Son commune (Da Bac district, Hoa binh province) and Quy Huong commune (Moc Chau district, Son la province) are remote mountainous areas. Methods as interview, stool examination by Kato technique, sputum examination and snail examination by direct technique, crab muscle digestion were used in the study. Results of the study, showed that rate of people having habit of eating raw crab was 73.0% in Toan Son and 72.2% in Quy Huong; paragonimiasis rates were 3.3-11.3% and 3.4-15.7%, Ascaris infection rates was 52.3% and 50.0%, hookworm infection rates was 33.0% and 35.9%, Trichuris infection rates was 29.7% and 4.3%, clonorchiasis infection rate was 0.7 % (in Toan Son only), Taenia infection rates was 0.4% and 0.3%.In these communes, dogs, crabs and snails were also infected with paragonimus.


Sujets)
Helminthiase , Épidémiologie
14.
Journal of Practical Medicine ; : 13-16, 2005.
Article Dans Vietnamien | WPRIM | ID: wpr-6428

Résumé

Since 2002, adult worms of human small liver fluke in some provinces were identified by morphology and confirmed by molecular method as Clonorchis sinensis in Thanh Hoa, Ninh Binh, Nam Dinh and Bac Giang province; Opisthorchis viverrini in Phu Yen and Binh Dinh province. This study, using polymerase chain reaction (PCR), a portion of the cytochrome oxidase 1 (410 nucleotide and 136 amino acid) of mitochondrial genome of adult worms of small liver fluke from human in Nghe An (CsNA) and Dong Nai (CsDN) province were amplified. The nucleotide and amino acid of these sequences was comparatively analyzed with the known Clonorchis sinensis (Nam Dinh strain). The analysis revealed that the Nghe An Clonorchis sp and Dong Nai Clonorchis sp (in human) were homology (99.5 - 99.8% nucleotide) and similar (100% amino acid) to the Nam Dinh Clonorchis sinensis (identification was done). Thus, the Nghe An and Dong Nai Clonorchis sp were identified as Clonorchis sinensis in Opisthorchiidae family, Clonorchis genus.


Sujets)
Humains , Helminthes , Trematoda
15.
Journal of Practical Medicine ; : 9-12, 2005.
Article Dans Vietnamien | WPRIM | ID: wpr-5725

Résumé

Using polymerase chain reaction (PCR), a portion of the cytochrome oxidase 1 (446 nucleotide and 148 amino acid) of mitochondrial genome of adult worm of small liver fluke from human in Hiep Hoa district, Bac Giang (CsNgBG) province was amplified. The nucleotide and amino acid of these sequences were comparatively analyzed with the known Clonorchis sinensis. The analysis revealed that the Bac Giang Clonorchis sp (in human) was similar (100% nucleotide and amino acid) to the Nam Dinh Clonorchis sinensis (identification was done); 99.6% (nucleotide) and 100% (amino acid) homology to the C.sinensis of Chinese strain (CsCN-Gen Bank AF 184619) and Korean strain (CsKor-Gen Bank AF 181889). Thus, the Bac Giang Clonorchis sp is identified as Clonorchis sinensis in Opisthorchidae family, Clonorchis genus. Phylogenetic analysis uniquely placed the Bac Giang Clonorchis sinensis to the group of Chinese and Korean Clonorchis sinensis.


Sujets)
Adulte , Helminthes , Fasciola hepatica
16.
Journal of Practical Medicine ; : 31-33, 2004.
Article Dans Vietnamien | WPRIM | ID: wpr-5069

Résumé

A survey concerning the infestation of clonorchis sinensis in human was conducted in 4 communes of Ninh Binh and Nam Dinh provinces, from each, 100 households with their members of above 6 years old of age were enrolled randomly. Results showed a prevalence of 31.3 – 37.3% in Nam Dinh and 22.5% in Ninh Binh epidemic areas. The prevalence in male subjects was higher than in female (45.5 –47.6% versus 10.9 – 26.1%). The age group of 40-49 years old had had the highest prevalence (51.9%). There, 35.9 – 80.4% of people had consumed raw fish.


Sujets)
Opisthorchis , Épidémiologie , Clonorchis sinensis
17.
Journal of Practical Medicine ; : 28-31, 2004.
Article Dans Vietnamien | WPRIM | ID: wpr-4993

Résumé

Analysis samples of meat and seafood... in Hanoi markets to determine infections of larva of parasites. Results: among 144,390 beef samples and 2,237,000 pork samples, prevalences of parasite infection were 0.027% and 0.003%, respectively. The rate of liver fluke metacercaria in fresh water fish was 1.7-21.7% (mean rate 5.2%), rate of Gnathostoma infection in snakehead fish and ell was 4.8-11.4%. Furthermore, in endemic areas of lung fluke, infection rate of Paragonimus metacercaria in fresh water crabs and fish was 9.7%-98.1% (mean rate 63%).


Sujets)
Contamination des aliments , Parasites , Aliments , Épidémiologie
18.
Journal of Practical Medicine ; : 42-46, 2004.
Article Dans Vietnamien | WPRIM | ID: wpr-4841

Résumé

A 21 -year -old woman patient, from middle of February 2003, she had got fatigue, weight loss 5-6 kg within 3 weeks, bad appetite, indigestion. Then she had pain in right lower rib, 390C fever, cough, dry vomitting. She had got diagnosis of hepatic abscess and unsuccessfully treated by 10 days antibiotics. She had got cecal abscess and abscess block was removed. Stool examination found Fasciola hepatica eggs and ELISA serum test was (+) with F.gigantica antigen (collected from buffaloes and cows) by title of 1/12800, acidophil white blood cell 12%. Triclabendazole 10mg/kg gave good results.


Sujets)
Fasciola , Vietnam , Oeufs , Génome mitochondrial
19.
Journal of Medical and Pharmaceutical Information ; : 13-16, 2004.
Article Dans Vietnamien | WPRIM | ID: wpr-4554

Résumé

There are various genera of taeniasis but in Vietnam Taenia saginata, Taenia solium and Taenia asiatica. Among them, only Taenia solium is currently identified as the cause of cysticercosis. Taeniasis and certicercosis are distributed largely. The incidence of taeniasis is 0.5-12.6%. Certicercosis is occured at least in 49 provinces and cities in whole of the country, with the incidence of 5.7% in Bac Ninh and 19.7% in Ho Chi Minh city. These conditions affect considerably on community health. In local health services, the treatment of cysticercosis is now difficult. At basic level the collaboration of health services and veterinary health is recommended in the control of tamiasis and certicercosis


Sujets)
Humains , Taeniase , Cysticercose , Épidémiologie
20.
Journal of Preventive Medicine ; : 99-104, 2004.
Article Dans Vietnamien | WPRIM | ID: wpr-4421

Résumé

The survival ability of the larve of Clonorchis sinensis in raw fish dish prepared by traditional culinary method in NghiaLac commune, NghiaHung District,NamDinh province and the larve of Paragonomus heterotrimus in grilled crabs processed in KhanhHoa commune, LucYen district, YenBai province was investigated..In this raw fish dish, 93-95% of the microcerca of Clonorchis sinensis and in this grilled crab, 23,3-65% of microcerca had been living


Sujets)
Poissons , Clonorchis sinensis , Épidémiologie
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