Detecting haplotypes of four Y-STR by multiplex amplification / 法医学杂志

OBJECTIVE@#To establish a multiplexing Y-STR system and study haplotype frequencies of 4 Y-specific loci in China Han population.@*METHODS@#DYS439, DYS390, GATA-A7.2 and DYS393 loci were amplified simultaneously and were analyzed by polyacrylamide gel electrophoresis and silver staining.@*RESULTS@#When 558 unrelated male individuals from the Han population in China were tested by the multiplex system, DYS439, DYS390, GATA-A7.2 and DYS393 show 7,7,7,6 alleles, respectively. 180 different haplotypes were detected. The power of discrimination of this system was 0.9853.@*CONCLUSION@#The multiplex amplified system of these 4 Y-specific loci and their database are useful for human origin exploration and forensic practice.