Quantitative model for patchouli alcohol in Pogostemon cablin by near-infrared spectroscopy / 中国中药杂志

<p><b>OBJECTIVE</b>To establish a near-infrared spectroscopy quantitative model for rapid determination of the patchouli alcohol content in Pogostemon cablin.</p><p><b>METHOD</b>The gas chromatography was adopted for determining the content of patchouli alcohol content in 102 batches of P. cablin samples. Their near-infrared spectrograms were collected and preprocessed by standard normal variate and the first derivative of Savitsky-Golay. The quantitative model of patchouli alcohol content was established by the partial least squares regression analysis.</p><p><b>RESULT</b>According to the correction model established in this study, the root-mean-square error of calibration, the root-mean-square error of prediction and the root-mean-square error of cross-validation of the calibration model for Patchouli alcohol were 0.991 10, 0.012 9, 0.012 8 and 0.033 15, respectively.</p><p><b>CONCLUSION</b>The near-infrared spectroscopy quantitative model established in this study is stable, accurate and reliable for the rapid determination of the content of patchouli alcohol in P. cablin.</p>

Study on HPLC Fingerprint of Desmodium styracifolium and Its Preparations / 中国药房

OBJECTIVE:To establish RP-HPLC fingerprints of Desmodium styracifolium and its preparations in order to provide basis for the quality evaluation of them.METHODS:The separation was performed on ODS-C18(250 mm?4.6 mm,5 ?m) column with mobile phase consisted of methanol-20% phosphoric acid(gradient elution).The detection wavelength was set at 360 nm and flow rate was 1.0 mL?min-1.Column temperature was set at 25 ℃.The RP-HPLC fingerprint of D.styracifolium and its preparations were recorded.RESULTS:There were 13 common peak in RP-HPLC fingerprint of D.styracifolium and its preparations.CONCLUSION:The method is accurate,stable and reproducible for basis of quality evaluation and RP-HPLC finger print of D.styracifolium and its preparations.