Résumé
Background: Resistant strains of Pseudomonas aeruginosa to imipenem was medical treatment problem, especially in burnt units of hospitals
Objectives: This study was conducted to evaluate the antimicrobial effect of Fe[2]O[3] nanoparticles alone and functionalized with imipenem on P. aeruginosa starins producing metallo beta-lactamases [MBL]
Materials and Methods: A disk diffusion method was used to isolate a clinical P. aeruginosa producing Metallo beta-lactamases with imipenem resistance. The minimum inhibitory concentration [MIC] of Fe[2]O[3] nanoparticles and imipenem were calculated against the bacteria. The antimicrobial effect of nanoparticles functionalized with the antibiotic was determined. Standard strain of P. aeruginosa ATCC: 27853 was used as control
Results: The clinical sample was resistant to imipenem [up to 28 micro g.mL[-1]]. Similarly, MIC of the nanoparticles against the isolate was 160 micro g.mL[-1]. Subsequently, the combination of 16 pg.mL[-1] of antibiotic with 80 micro g.mL[-1] of Fe[2]O[3] nanoparticles were able to inhibit the growth of the isolate
Conclusions: Fe[2]O[3] nanoparticles functionalized with imipenem can impair antibiotic resistance mechanisms of bacteria as it can make the imipenem resistant the aforementioned bacterium more susceptible to weaker concentrations of antibiotic. It also has its own antibacterial effect in certain concentrations