Résumé
We describe a "sandwich" enzyme-linkedimmunosorbent assay (ELISA) sensitive to quantities of scorpion (Tityus serrulatus) venom (TsV) in the range of 1-3 ng?ml sample. Cross-reactivity with the venom from the rattlesnake Crotalus durissus terrificus and with venoms from several snakes of the Bothrops genus was detected only at concentrations higher than 1 *g/ml sample. A conventional ELISA is also described for the detection of antibodies against TsV. Analysis by Western Blot (WB) demonstrated a 25-kDa protein band common to TsV and to the venoms of Bothrops moojeni, B. jararacussu and B. jararaca. Venom from C. d. terrificus exhibited WB cross-reactive bands of 16 and 25 kDa with TsV
Sujets)
Lapins , Animaux , Mâle , Immunisation , Immunoglobuline G/analyse , Venins de scorpion/immunologie , Technique de Western , Chromatographie d'affinité , Réactions croisées , Venins de crotalidé/immunologie , Test ELISARésumé
Tityustoxin (TsTx), the purified venom of the Brazilian scorpion Tityus serrulatus, was inected intravenously (50 microng/Kg) into rats, producing a typical picture of chronic pancreatitis after 20 days. Nesidioblastosis, a lesions characterized by hyperplasia of the islets of Langerhans, was also detected in a high percentage (40%) of animals. TsTx-induced pancreatitis may be a useful model for the study of nesidioblastosis in laboratory animals
Sujets)
Rats , Animaux , Mâle , Neurotoxines/toxicité , Maladies du pancréas/anatomopathologie , Pancréatite/induit chimiquement , Venins de scorpion/toxicité , Maladie chronique , Injections veineuses , Pancréatite/anatomopathologie , Venins de scorpion/administration et posologieRésumé
Relata-se poliarterite nodosa na infancia associada a endocardite infecciosa descrita em seus aspectos clinicos laboratoriais e histopatologicos.Discutem-se as dificuldades de diagnostico, etiopatogenia, assim como o envolvimento neurologico central e a resposta a terapeutica instituida.com dois modelos e resistencia, sensive