Your browser doesn't support javascript.
loading
Montrer: 20 | 50 | 100
Résultats 1 - 3 de 3
Filtre
1.
Arq. bras. med. vet. zootec ; 68(4): 1095-1098, jul.-ago. 2016. tab, ilus
Article Dans Portugais | LILACS, VETINDEX | ID: biblio-868454

Résumé

The aim of this study was to verify whether enalapril and captopril would reverse the renal damage caused by N-methylglucamine antimoniate in C57BL/6 mice. We used inbred C57BL/6 female mice, obtained from the Oswaldo Cruz Foundation (FIOCRUZ), Salvador, BA. The mice were divided into four groups as follows: Group1: received saline by the intramuscular (IM) route; Group 2: received N-methylglucamine antimonate (IM); Group 3: received N-methylglucamine antimoniate and captopril; Group 4: was treated with N-methylglucamine antimoniate and enalapril. Both enalapril and captopril were orally administered in drinking water (ad libitum). After 30 days of treatment, the animals were sacrificed and their kidneys were collected for histological analysis which showed that enalapril completely reversed the edema, the podocytes hyperplasia and nucleus of the epithelial cells in the proximal convoluted tubules caused by N-methylglucamine antimoniate. On the other hand, the captopril treatment partially inhibited kidney harmful effects caused by N-metilgucamina antimoniate. Taken together, we would conclude that enalapril and captopril reverse edema and renalhyperplasia caused by N-methylglucamine antimonate in mice.(AU)


Sujets)
Animaux , Femelle , Cochons d'Inde , Souris , Atteinte rénale aigüe/induit chimiquement , Inhibiteurs de l'enzyme de conversion de l'angiotensine/usage thérapeutique , Effets secondaires indésirables des médicaments/médecine vétérinaire , Leishmaniose/médecine vétérinaire
2.
Braz. j. med. biol. res ; 44(3): 212-216, Mar. 2011. ilus
Article Dans Anglais | LILACS | ID: lil-576066

Résumé

Keratinases are enzymes of great importance involved in pathogenic processes of some fungi. They also have a widespread ecological role since they are responsible for the degradation and recycling of keratin. On the one hand, studying them furthers our knowledge of pathogenicity mechanisms, which has important implications for human health, and on the other hand, understanding their ecological role in keratin recycling has biotechnological potential. Here, a wild-type keratinolytic Candida parapsilosis strain isolated from a poultry farm was treated with ethyl methanesulfonate in order to generate mutants with increased keratinase activity. Mutants were then cultured on media with keratin extracted from chicken feathers as the sole source of nitrogen and carbon. Approximately 500 mutants were screened and compared with the described keratinolytic wild type. Three strains, H36, I7 and J5, showed enhanced keratinase activity. The wild-type strain produced 80 U/mL of keratinolytic activity, strain H36 produced 110 U/mL, strain I7, 130 U/mL, and strain J5, 140 U/mL. A 70 percent increase in enzyme activity was recorded for strain J5. Enzymatic activity was evaluated by zymograms with proteic substrates. A peptidase migrating at 100 kDa was detected with keratin, bovine serum albumin and casein. In addition, a peptidase with a molecular mass of 50 kDa was observed with casein in the wild-type strain and in mutants H36 and J5. Gelatinase activity was detected at 60 kDa. A single band of 35 kDa was found in wild-type C. parapsilosis and in mutants with hemoglobin substrate.


Sujets)
Animaux , Candida/enzymologie , Peptide hydrolases/métabolisme , Candida/effets des médicaments et des substances chimiques , Candida/physiologie , Électrophorèse sur gel de polyacrylamide , Méthanesulfonate d'éthyle/pharmacologie , Mutagènes/pharmacologie , Mutation/génétique , Volaille , Spécificité du substrat
3.
In. Schiabel, Homero; Slaets, Annie France Frère; Costa, Luciano da Fontoura; Baffa Filho, Oswaldo; Marques, Paulo Mazzoncini de Azevedo. Anais do III Fórum Nacional de Ciência e Tecnologia em Saúde. Säo Carlos, s.n, 1996. p.391-392.
Monographie Dans Portugais | LILACS | ID: lil-236409

Résumé

Atualmente, os hospitais tem demonstrado maior interesse no Programa de Garantia de Qualidade, bem como, na Radioproteção, no que tange a serviços que envolvam radiações ionizantes. Faz-se necessário, perante a esta tendência, a presença do Físico Médico, que irá elaborar plano de Proteção Radiológica, Programa de Garantia da Qualidade informar mensalmente as doses individuais, acompanhar inspeções sanitárias, treinar e orientar os profissionais envolvidos nessa área. Portanto, o presente trabalho tem o objetivo de relatar as atividades desempenhadas por este profissional nos Serviço de Radiologia, Hemodinâmica e unidade de Bloco Cirúrgico, assim como, a metodologia e os resultados obtidos.


At present, the hospitals have showed more interest in the Quality Assurance Program, also, in Radioprotection, about the services that involve ionization radiation. This is necessery, because a treemd, the presence of the Medical Physics, that will organize a Radiologic Protection project, Quality Assurance Program, inform monthly the individual doses, accompany sanitary inspection, train and orientale the profissionals involve in this area. Consequently, the present paper has the; objective to give an account of the activities executed for this profissional in the Radiologic, Hemodinamic and Surgery Unit, also, the metodology and the results obteined with it.


Sujets)
Département hospitalier de chirurgie , Hémodynamique , Médecine physique et de réadaptation , Service hospitalier de radiologie-radiothérapie , Contrôle de qualité , Radiométrie , Brésil , Radioprotection/méthodes , Rayonnement ionisant
SÉLECTION CITATIONS
Détails de la recherche