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Rev. argent. microbiol ; 33(1): 28-35, ene.-mar. 2001.
Article Dans Anglais | LILACS | ID: lil-332504

Résumé

Effects of bovine mastitis pathogen virulence factors on mammary epithelial cell function are not clearly understood. In this study, the effect of streptococcal lipoteichoic acid (LTA), streptokinase, and Escherichia coli lipopolysaccharide (LPS) on proliferation of a primary bovine mammary epithelial cell culture (BTE) and on an established bovine mammary epithelial cell line (MAC-T) was evaluated. Mammary epithelial cells were cultured in the presence of bacterial virulence factors for 48 h at 37 degrees C. BTE cell proliferation was inhibited by streptococcal LTA at 8 and 16 micrograms/ml whereas MAC-T cell proliferation was reduced significantly by concentrations of LTA > or = 2 micrograms/ml. Streptokinase had no effect on proliferation of either MAC-T or BTE cells and LPS inhibited proliferation of BTE but not of MAC-T cells. Effect of LTA and LPS on mammary epithelial cell proliferation could be relevant during the periparturient period when mammary glands are markedly susceptible to new intramammary infection and when mammary epithelial cells undergo extensive proliferation, differentiation and synthesis of milk components.


Sujets)
Animaux , Bovins , Femelle , Grossesse , Acides teichoïques/toxicité , Cellules épithéliales/effets des médicaments et des substances chimiques , Escherichia coli , Lipopolysaccharides/toxicité , Glandes mammaires animales , Mammite bovine , Streptococcus , Streptokinase , Acides teichoïques/pharmacologie , Lignée de cellules transformées , Cellules cultivées , Clones cellulaires , Division cellulaire/effets des médicaments et des substances chimiques , Techniques immunoenzymatiques , Infections à streptocoques/microbiologie , Infections à streptocoques/médecine vétérinaire , Infections à Escherichia coli/microbiologie , Infections à Escherichia coli/médecine vétérinaire , Kératines , Lipopolysaccharides/pharmacologie , Marqueurs biologiques , Vimentine , Virulence
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