Biochemical and pathogenic classification of candida albicans

Candida albicans is the yeast most frequently isolated as a commensal or as a pathogen. Yet its epidemiological study is difficult because of the lack of a strict typing system; although many trials were made. We aim to reveal any degree of difference in the biochemical and or the pathogenic characters of those strains isolated as a commensal and those isolated from candidosis C. albicans is the yeast most frequently isolated [75.3% of all yeasts]. Its identification can be successfully done by two simple tests: failure to grow on MacConkey's agar and tolerance to pH 1.55. Biotyping system is not statistically significant while most criteria of pathogenicity are significantly different in commensal and pathogenic strains:% of chlamydospores on RMA, growth kinetics, adherence capability, gelatin hydrolysis and morphology of giant colonies

Rapid grower mycobacteria in pulmonary tuberculosis

Mycobacterium fortuitum and Mycobacterium cheloni are two species of the M. fortuitum complex presently considered to be pathogenic for humans. Out of 60 suspected cases of pulmonary tuberculosis with no previous antituberculous treatment, there were 17 strains [28.33%] of rapid growers acid fast bacilli, showed growth on Lowenstein-Jensen mediun within 7 days at 27degree. Using biochemical reactions [catalase, arylsulfatase, nitrate reduction, iron uptake, tellurite reduction] and growth on MacConkey agar without crystal violet, they were distinguished into 9 strains [52.94%] M. fortuitum, 5 strains [29.41%] M. cheloni and 3 strains [17.64%] M. phlei