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1.
J. venom. anim. toxins incl. trop. dis ; 13(1): 56-68, 2007. ilus
Article Dans Anglais | LILACS | ID: lil-444611

Résumé

Snake venoms are toxic to a variety of cell types. However, the intracellular damages and the cell death fate induced by venom are unclear. In the present work, the action of the South American rattlesnake Crotalus durissus terrificus venom on CHO-K1 cell line was analyzed. The cells CHO-K1 were incubated with C. d. terrificus venom (10, 50 and 100g/ml) for 1 and 24 hours, and structural alterations of actin filaments, endoplasmic reticulum and nucleus were assessed using specific fluorescent probes and agarose gel electrophoresis for DNA fragmentation. Significant structural changes were observed in all analyzed structures. DNA fragmentation was detected suggesting that, at the concentrations used, the venom induced apoptosis.


Sujets)
Animaux , Mâle , Femelle , Cellules CHO , Réticulum endoplasmique , Venins de crotalidé , Apoptose
2.
Biocell ; 27(3): 301-309, Dec. 2003.
Article Dans Anglais | LILACS | ID: lil-384240

Résumé

The effects of Photodynamic Therapy using 2nd generation photosensitizers have been widely investigated aiming clinical application treatment of solid neoplasms. In this work, ultrastructure changes caused by the action of two 2nd generation photosensitizers and laser irradiation on CHO-K1 and HeLa (neoplastic) cells were analyzed by transmission electron microscopy. Aluminum phthalocyanine chloride, aluminum phthalocyanine tetrasulfonate chloride and radiation from a semiconductor laser at a fluency of 0.5 J/cm2 (Power=26 mW; lambda=.670 nm) were used. The results showed induction of apoptosis. Such alterations where observed in HeLa but not in CHO-K1 cells after Aluminum phthalocyanine tetrasulfonate chloride (AlPcS4, photodynamic treatment. The Aluminum phthalocyanine chloride (AlPc) photodynamic treatment induced necrosis on the neoplastic cell line, and cytoplasm and nuclear alterations on the normal cell line.


Sujets)
Humains , Femelle , Cricetinae , Photothérapie dynamique/méthodes , Lasers , Tumeurs du col de l'utérus/traitement médicamenteux , Ovaire/effets des médicaments et des substances chimiques , Radiosensibilisants/pharmacologie , Apoptose/effets des médicaments et des substances chimiques , Apoptose/physiologie , Apoptose/effets des radiations , Cellules CHO , Cytoplasme/effets des médicaments et des substances chimiques , Cytoplasme/effets des radiations , Cytoplasme/ultrastructure , Composés organométalliques/pharmacologie , Stimulation lumineuse/instrumentation , Stimulation lumineuse/méthodes , Cellules HeLa , Indoles/pharmacologie , Lumière , Microscopie électronique , Mitochondries/effets des médicaments et des substances chimiques , Mitochondries/effets des radiations , Mitochondries/ultrastructure , Nécrose , Tumeurs du col de l'utérus/ultrastructure , Noyau de la cellule/effets des médicaments et des substances chimiques , Noyau de la cellule/effets des radiations , Noyau de la cellule/ultrastructure , Ovaire/ultrastructure
3.
Braz. j. med. biol. res ; 25(10): 1015-24, 1992. tab, graf
Article Dans Anglais | LILACS | ID: lil-134645

Résumé

1. Ingestion of enteropathogenic Escherichia coli or Candida albicans by thioglycollate-elicited macrophages and polymorphonuclear leukocytes was investigated in vitro, 2. Goat antiserum against mannose receptors caused about 50% inhibition of E. coli phagocytosis and about 90% inhibition of C. albicans phagocytosis. 3. E. coli and C. albicans uptake was inhibited by about 60% and 98%, respectively, by plating the macrophages onto substrates coated with poly-L-lysine-mannan. Further addition of 50 mM mannose to the medium significantly increased the inhibition of phagocytosis of E. coli by macrophages from 60.7 +/- 1.5 to 79.8 +/- 13.1 and by polymorphonuclear cells from 58.9 +/- 3.7 to 88.7 +/- 4.9. 4. Preincubation of phagocytic cells with antiserum against substance A of human erythrocytes reduced E. coli ingestion by 95%, but this inhibition was not observed when the antiserum was incubated with N-acetylgalactosamine (50 mM) before being added to the phagocytes. The phagocytosis of C. albicans was not inhibited by anti-substance A antiserum. 5. The phagocytosis of E. coli was inhibited by about 25% by the addition of 7.8 micrograms/ml soluble mannan to the medium, and by about 50% by the addition of 50 mMN-acetylgalactosamine; when both substances were added to the medium, an additive inhibition of about 75% was observed. 6. These results indicate that mannose receptors on the surface of phagocytic cells mediate E. coli or Candida albicans uptake and that the binding of bacteria to N-acetylgalactosamine residues from the membrane of phagocytes is also involved in the phagocytosis of E. coli


Sujets)
Animaux , Humains , Candida albicans/immunologie , Escherichia coli/immunologie , Phagocytose/immunologie , Récepteur mitogène/immunologie , Acétyl-galactosamine/pharmacologie , Adhérence bactérienne/effets des médicaments et des substances chimiques , Adhérence bactérienne/immunologie , Candida albicans/effets des médicaments et des substances chimiques , Candida albicans/pathogénicité , Dépression chimique , Érythrocytes/effets des médicaments et des substances chimiques , Érythrocytes/immunologie , Escherichia coli/effets des médicaments et des substances chimiques , Escherichia coli/pathogénicité , Sérums immuns/pharmacologie , Macrophages péritonéaux/effets des médicaments et des substances chimiques
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