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1.
The Korean Journal of Parasitology ; : 61-70, 2018.
Article Dans Anglais | WPRIM | ID: wpr-742221

Résumé

We developed a Rapid Diagnostic Test (RDT) kit for detecting IgG/IgM antibodies against Zika virus (ZIKV) using monoclonal antibodies to the envelope (E) and non-structural protein 1 (NS1) of ZIKV. These proteins were produced using baculovirus expression vector with Sf9 cells. Monoclonal antibodies J2G7 to NS1 and J5E1 to E protein were selected and conjugated with colloidal gold to produce the Zika IgG/IgM RDT kit (Zika RDT). Comparisons with ELISA, plaque reduction neutralization test (PRNT), and PCR were done to investigate the analytical sensitivity of Zika RDT, which resulted in 100% identical results. Sensitivity and specificity of Zika RDT in a field test was determined using positive and negative samples from Brazil and Korea. The diagnostic accuracy of Zika RDT was fairly high; sensitivity and specificity for IgG was 99.0 and 99.3%, respectively, while for IgM it was 96.7 and 98.7%, respectively. Cross reaction with dengue virus was evaluated using anti-Dengue Mixed Titer Performance Panel (PVD201), in which the Zika RDT showed cross-reactions with DENV in 16.7% and 5.6% in IgG and IgM, respectively. Cross reactions were not observed with West Nile, yellow fever, and hepatitis C virus infected sera. Zika RDT kit is very simple to use, rapid to assay, and very sensitive, and highly specific. Therefore, it would serve as a choice of method for point-of-care diagnosis and large scale surveys of ZIKV infection under clinical or field conditions worldwide in endemic areas.


Sujets)
Anticorps , Anticorps monoclonaux , Baculoviridae , Brésil , Réactions croisées , Virus de la dengue , Diagnostic , Tests diagnostiques courants , Test ELISA , Flavivirus , Or colloïdal , Hepacivirus , Immunoglobuline G , Immunoglobuline M , Corée , Méthodes , Tests de neutralisation , Systèmes automatisés lit malade , Réaction de polymérisation en chaîne , Trousses de réactifs pour diagnostic , Sensibilité et spécificité , Cellules Sf9 , Fièvre jaune , Virus Zika
2.
Journal of the Korean Society of Virology ; : 165-174, 1999.
Article Dans Coréen | WPRIM | ID: wpr-27130

Résumé

To investigate viral pathogenesis and in vivo efficacy of acyclovir (ACV) in mouse HSV-1 encephalitis models, female BALB/c mice aged 5 weeks were inoculated with strain F either intranasally (IN) or intracerebrally (IC). ACV-treatment by intraperitorneal injection with 0, 5, 10 and 25 mg/kg b.i.d. for 6days was commenced 1 h after infection. Body weight and signs of clinical disease were noted daily up to 2 weeks. ED50 of ACV in IN infection was 5mg/kg and 14.1 mg/kg in IC infection. Tissues of cental nervous system were collected from 2 mice per group everyday up to 5 days p.i. and the virus titers were measured. In IN infection model, high titers in eyes and trigeminal nerves were observed. ACV-treatment showed significant reduction of the titers in all the isolated. In IC infection model, cerebrum, cerebellum and brain stem showed high virus titers. ACV-treatment showed less significant reduction of virus titers than that in IN infection model. Reactivation of explanted trigeminal nerves from mice 30 day p.i. was monitored. In all of ACV treated mice reactivation was observed, i.e. even the highest dose of ACV did not inhibit the establishment of viral latency.


Sujets)
Animaux , Femelle , Humains , Souris , Aciclovir , Poids , Tronc cérébral , Cervelet , Cerveau , Encéphalite , Herpès , Herpèsvirus humain de type 1 , Système nerveux , Simplexvirus , Nerf trijumeau , Latence virale
3.
Journal of the Korean Society of Virology ; : 175-182, 1999.
Article Dans Coréen | WPRIM | ID: wpr-27129

Résumé

An attractive target for anti-herpes chemotherapy is the herpes simplex virus 1 (HSV-1) protease encoded by the UL26 gene. HSV-1 protease is essential for DNA packaging and virus maturation. To perform high throughput for potent inhibitors, the efficient production of larger amounts of highly purified enzyme and protease activity assay method must be established. In this report, expression in E. coli and purification of the protease gene of HSV-1 strain F was investigated. The protease gene was cloned pET28, and the nucleotide sequence of protease catalytic domain of HSV-1 compared strain F with other strains (KOS and CL101). In these results the F strain was different in base sequence. However, the amino acid sequence was identifical. The HSV-1 protease was purified with His-tagged affinity column. The analysis of HSV-1 protease activity Was performed by high performance liquid chromatography.


Sujets)
Séquence d'acides aminés , Séquence nucléotidique , Domaine catalytique , Chromatographie en phase liquide à haute performance , Chromatographie en phase liquide , Clones cellulaires , Empaquetage de l'ADN , Traitement médicamenteux , Herpès , Herpèsvirus humain de type 1 , Simplexvirus
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