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Rev. Inst. Med. Trop. Säo Paulo ; 45(6): 339-342, Nov.-Dec. 2003. ilus, tab
Article Dans Anglais | LILACS | ID: lil-353986

Résumé

In the present study, the performance of Immunomagnetic Separation technique, coupled with Immunofluorescence (IMS-IFA), was compared with the FAUST et al. and Lutz parasitological techniques for the detection of Giardia lamblia cysts in human feces. One hundred and twenty-seven samples were evaluated by the three techniques at the same time showing a rate of cyst detection of 27.5 percent by IMS-IFA and 15.7 percent by both Faust et al. and Lutz techniques. Data analysis showed a higher sensitivity of IMS-IFA for the detection of G. lamblia cysts in comparison with the techniques of FAUST et al. and Lutz. The use of this methodology as a routine procedure enables the processing of many samples simultaneously, in order to increase recovery rate of G. lamblia cysts and reduce the time of sample storage.


Sujets)
Animaux , Humains , Enfant d'âge préscolaire , Enfant , Giardia lamblia , Giardiase , Fèces , Technique d'immunofluorescence , Giardia lamblia , Séparation immunomagnétique , Sensibilité et spécificité
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