Plant Growth Promotion and Root Colonization by EPS Producing Enterobacter sp. RZS5 under Heavy Metal Contaminated Soil.

The heavy metal resistant bacterium isolated from field soil and identified as Enterobacter sp. RZS5 tolerates a high concentration (100-2000 mM) of various heavy metal ions such as Mn2+, Ni2+, Zn2+, Cu2+, CO2+ and Fe2+ when grown in such environment and produces exopolysaccharides (EPS). Here, we have demonstrated EPS production by Enterobacter sp. RZS5 during 60 h of growth in yeast extract mannitol broth (YEMB). The yield increased by two fold after the addition of 60 M of Ca2+; 50 M of Fe2+ and 60 M of Mg2+ ions in YEMB, and the optimization of physico-chemical parameters. EPS was extracted with 30% (v/v) of isopropanol as against the commonly used 50% (v/v) isopropanol method. EPS-rich broth promoted seed germination, shoot height, root length, number of leaves and chlorophyll content of wheat (Triticum aestivum) and peanut (Arachis hypogaea) seeds. The higher colony-forming unit of Enterobacter sp. in soil inoculated with EPS rich broth of Enterobacter sp. indicated the root colonizing potential and rhizosphere competence of the isolate. The FTIR spectra of the EPS extract confirmed the presence of the functional group characteristics of EPS known to exhibit a high binding affinity towards certain metal ions. This overall growth and vigour in plants along with the effective root colonization, reflected the potential of the isolate as an efficient bio-inoculant in bioremediation.

A Postmortem Study of Pedestrian Injuries to Find out Risk Factors And Preventive Measures.

Objectives: Present study is a little effort to understand factors responsible for pedestrians accidents, to determine risk factors, sources and causes of mortality involved in pedestrians accidents, to reach a conclusion regarding prevention of pedestrians accidents and reducing pedestrians mortality. Materials and Method: Present study is a retrospective study based on an analysis of 156 autopsies on cases of pedestrian accidents at Sheth V.S. General Hospital, Ahmadabad during two consecutive years from May 2008 to April 2010. Result: Following risk factors are identified from this study- old age, heavy motor vehicles, city life, morning hours of the day etc. Pedestrians are the most vulnerable to vehicular injuries and victims of road traffic accidents. There is less awareness in pedestrians as well as in drivers of vehicles regarding road traffic accidents and its consequences.

A Study of Pattern of Vehicular Injuries over Pedestrians: A Retrospective Study.

Objectives: Present study makes a little effort to understand patterns of injuries over pedestrians during road traffic accidents, to set on record a statistical statement of the severity and survival period in pedestrians accidents and to define, delineate and compare present study with other available studies. Materials and Methods: Present study is based on an analysis of 156 autopsies on victims of pedestrian accidents at Sheth V.S. General Hospital, Ahmedabad during two consecutive years from May 2008 to April 2010. Result: This study shows the males (80.8%) are outnumbered the females (19.2%). Evaluation of patterns of injuries shows head injury is overall most common in pedestrians. In primary impact injury head injury (41%) and lower limb injuries (36.5%) are most common followed by pelvic injuries (12.1%). Head injury (33.9%) is again most common in secondary impact injuries. Chest injuries (16.6%) and abdomen injury (12.8%) are most common secondary injuries to pedestrians followed by head (8.3%) and neck (5.1%). Road traffic accidents are the major contributors of unnatural deaths worldwide. Pedestrians are the most commonly involved victims in such cases.

Production of biocontrol traits by banana field fluorescent Pseudomonads and comparison with chemical fungicide.

Pseudomonas aeruginosa isolated from banana field rhizosphere produced different antifungal metabolites like bactriocin, hydrogen cyanide and siderophore. Bacteriocinogenic, siderophoregenic, and HCN rich broth of isolate inhibited the growth of phytopathogen like Aspergilus niger, Aspergilus flavus, Fusarium oxysporum and Alternaria alternata. The isolate exhibited more antifungal activity and comparatively low MIC vis-a-vis commonly used copper based systemic chemical fungicide;bil cop.