Résumé
Abstract The aim of this present study was to localize alveolar macrophages, Proliferating cell nuclear antigen (PCNA), Smooth muscle actin (SMA), Vimentin and Vasculo-endothelial growth factor (VEGF) in the tracheal epithelium and lung alveoli in three important breeds of goats namely, Pashmina, Bakerwali and non-descript goats by standard immunohistochemical methods. Large pulmonary macrophages were observed in the lung sections of all the three breeds of goat. Macrophages were observed in the alveolar septa and airway epithelium. The mean macrophage count for Pashmina, Bakerwali and non-descript goat were recorded as 23.4±1.47, 27.8±0.68 and 21.4±0.70, respectively. The PCNA activity was intense in the alveolar epithelial cells of lungs in Pashmina and Bakerwali goats and very high in the lung tissue of non-descript goat. The PCNA activity was intense in the bronchial epithelial cells of lungs in Bakerwali goat. Strong reaction of SMA was observed in the submucosa of bronchioles of Pashmina and Bakerwali goats. The submucosa of the tracheal tissue showed a strong reaction of SMA in the non-descript goat and moderate in the submucosa of trachea in the Bakerwali goat. A number of vimentin-positive cells were found in the lung parenchyma in both Pashmina and Bakerwali goats. Also, there was a strong reaction of vimentin mainly in submucosal area in non-descript goat. The VEGF activity was found to be intense in the lung alveoli of Bakerwali goat and strong in Pashmina and non-descript goats. The tracheal epithelium was moderately reactive to VGEF in all the three goats.
Résumé
The present study was conducted on buffalo foetuses (n=19) ranging from 7.4 (62 days) to 108 cm (317 days) curved crown rump length (CVRL) to study the distribution of phosphatases and oxidoreductases in thyroid gland during prenatal development. A progressive increase in phosphatase activity from 12.50 cm CVRL (85 days) to 41 cm CVRL (166 days) was observed around the blood vessels in the developing thyroid. Mild activity of Succinate dehydrogenase (SDH) was observed at 13.50 cm CVRL (89 days) whereas Lactate Dehydrogenase (LDH) activity was absent at this stage. SDH activity was correlated with mitochondrial localization in developing thyroid. Weak to moderate LDH activity was observed at 56 cm CVRL (200 days) suggesting the presence of glycolytic pathway in developing thyroid. Mild to moderate Reduced Nicotinamide Adenine Dinucleotide diaphorase (NADH-diaphorase) and Reduced Nicotinamide Adenine Dinucleotide Phosphate Diaphorase (NADPH-diaphorase) was noticed in the follicular cells and interfollicular spaces of the thyroid gland at 12.50 cm CVRL (85 days) which progressively increased with gestational age and became intense at 56 cm CVRL (200 days) indicating increase in metabolic activity.
Résumé
The tissue distribution of estrogen receptor alpha (ERα) and progesterone receptor (PR) was examined using immunohistochemical technique. Image analysis was done to quantify the immune reactivity. The ERα and PR was localized in luminal epithelium, glandular epithelium, stromal cells, endothelial cells and myometrium and few cells in perimetrium. The immuno staining was observed in the nuclei of cells, however, faint cytoplasmic staining for PR was also observed. Variations were seen in the different tissue compartments of the uterus and during the different phases of the estrous cycle. Significantly higher number of ERα positive cells was observed in lamina epithelialis as compared to stromal cells and smooth muscle cells in myometrium. Significantly higher percentage of ERα positive cells was observed in the lamina epithelialis and lining epithelium of endometrial glands during follicular phase as compared to the luteal phases of estrous cycle (P < 0.05). Higher number of PR positive cells was observed in lamina epithelialis as compared to stromal cells and smooth muscle cells in myometrium (P < 0.05). Higher percentage of PR positive cells was observed in the lamina epithelialis during follicular phase as compared to the luteal phases of estrous cycle (P < 0.05). There was no significant difference in the immuno reactivity in stromal cells, lining epithelium of endometrial glands and smooth muscle cells of myometrium during the phases of estrous cycle. The study concluded that ERα and PR expressions were higher during follicular phase as compared to the luteal phase