Enhancing Growth and Yield of Parching Sorghum through Organic Manure Application

Aims: The combination of various sources of organic manures along with liquid organic manures is important to maintain nutrient availability to crop and sustain higher level of soil fertility. The aim is to meet the nutrient demand of parching sorghum as per requirement at different stages through solid and liquid organic manures which can give greater productivity of crops.Study Design: The experiment was laid in Randomized Complete Block Design with 9 treatments replicated thrice.Place and Duration of Study: A field experiment was conducted at College of Agriculture, Vijayapur, during Rabi, 2020-21.Methodology: The treatments were T1-Ghanajeevamrutha based on 100% RDN (recommended dose of Nitrogen) as basal dose, T2-Vermicompost based on 100% RDN as basal dose, T3- T1 + foliar application of 10% vermiwash at 20 & 45 DAS (days after sowing), T4- T1 + foliar application of 10% cow urine at 20 & 45 DAS, T5- T1 + foliar application of 25% jeevamrutha at 20 & 45 DAS, T6- T2 + foliar application of 10% vermiwash at 20 & 45 DAS, T7- T2 + foliar application of 10% cow urine at 20 & 45 DAS, T8- T2 + foliar application of 25% jeevamrutha at 20 & 45 DAS and T9- Organic RPP of parching sorghum with SMJ-1 variety of parching sorghum.Results: The application of ghanajeevamrutha based on 100 per cent RDN as basal dose + foliar application of 25 per cent jeevamrutha at 20 and 45 days after sowing recorded significantly higher dry matter production (55.86 g plant-1), leaf area index (3.49), raw and roasted grain weight (39.49 g and 51.73 g respectively), raw and roasted grain yield (1063 kg ha-1 and 1505 kg ha-1 respectively) of parching sorghum (hurda) at harvest as compared to other treatments. In addition to improvement in the major plant nutrient uptake were recorded with incorporation of ghanajeevamrutha or vermicompost along with the liquid organic manure.Conclusion: The application of ghanajeevamrutha based on 100 per cent RDN as basal dose coupled with foliar application of 25 per cent jeevamrutha at 20 and 45 days after sowing recorded significantly higher growth, yield, quality and plant nutrient uptake by parching sorghum.

Serum lactate as a prognostic marker in patients with sepsis: a prospective study

Background: A prospective study to estimate the serum lactate levels and as a prognostic marker in patients with sepsis.Methods: 170 patients admitted with sepsis in B.L.D.E. (Deemed to be University) Shri. B. M. Patil Medical College, Hospital and Research Centre, Vijayapur from October 2014 to June 2016.Results: In this study the mean serum lactate value of first sample in survivors (146 patients) is 3.8±1.2 and non-survivors (24 patients) is 6.2±1.9 with p value<0.001 which is significant. The serum lactate value of the second sample in survivors (146) is 2.7±1.0 and in non survivors (24) is 6.3±1.8 with p value<0.001 which is significant. The mean value of serum lactate 1st sample collected at the time of admission is 4.1±1.6 and the mean value of serum lactate second sample collected at 24 - 48 hours after admission is 3.1±1.6.Conclusions: Lactate level more than 4 mmol/l, patients are at highest risk of mortality and an aggressive resuscitation strategy shall be warranted. Hence serum lactate is considered as an independent and significant prognostic marker in patients with sepsis and evaluates the treatment outcome.

Osteopetro-Rickets: A Rare Paradoxical Association in an Infant.

Infantile malignant osteopetrosis is a hereditary bone disease with intense positive balance of body calcium. Osteopetro-rickets is a very rare paradoxical association of infantile osteopetrosis and rickets. This is a case report of an infant with osteopetro- rickets. He presented with severe anaemia, splenomegaly, hepatomegaly and clinical signs of rickets. The clinical, biochemical and skeletal survey showed osteopetrosis and rickets. We also describe the pathophysiologic mechanism and various management options.

Control and prevention of rat fever (Leptospirosis) outbreak in six villages of Raichur district, Karnataka.

The study describes an attempt to record leptospirosis in Raichur taluk/district, in Karnataka, by serological test such as microscopic test and IgM by ELISA. A house to house survey was done in six villages of Krishna river basin. Cases were interviewed regarding chief complaints like, fever, headache and oliguria, age, sex and treatment given with diclofenac, doxycycline, tetracycline and vitamin B complex. A total of 1516 cases were treated. The age of the patients ranged between 10 and 71 years. Most of them were agricultural workers. Out of 15 blood samples 12 samples sent to DHO Office, 3 were negative for leptospirosis, the density of rats were increased in all villages, the mode of transmission was food and water contamination, duration of incubation period was 5 to 10 days. Health education, for the community was undertaken to take measures against rats. Causative agent may be Leptospirosis interrogans, 3 cases were referred to Civil Hospital, Raichur and Navodaya Medical College, Raichur. Community participation was excellent. This study showed the presence of leptospirosis among the community of 6 villages, the disease is posing health hazards for agricultural workers in Raichur taluka villages.

Effect of substrate feeding on production of fructosyltransferase by Penicillium purpurogenum

Penicillium purpurogenum was found to produce both extracellular and intracellular fructosyltransferase. The organism could also produce sucrose hydrolytic enzyme. Sucrose was found to be the best carbon source for fructosyltransferase production. Maximum intracellular and extracellular fructosyltransferase production was observed after 3rd and 4th day of cultivation, respectively. The enzyme activity was optimum at temperature of 55°C and pH 5.5. The addition of amino acids, like leucine induced slightly the extracellular fructosyltransferase production, where as histidine and leucine had little inductive effect on intracellular fructosyltransferase production. Enhanced production of fructosyltransferase by Penicillium purpurogenum was observed when sucrose content was restored by additional sucrose feeding to the cultivation medium during production period.

Penicillium purpurogenum foi identificado como produtor extracellular e intracelular de frutosiltransferase. O microrganismo também é capaz de produzir uma enzima hidrolítica de sacarose. Sacarose foi identificada como a melhor fonte de carbono para a produção de frutosiltransferase. A produção máxima de frutosiltransferase extracelular e intracelular foi observada após o 3° e 4° dia de cultivo, respectivamente. Atividade ótima da enzima foi observada na temperatura de 55° C e pH 5,5. A adição de amino ácidos, como leucina, induziu ligeiro aumento na produção extracelular de frutosiltransferase, enquanto que histidina e leucina induziram um pequeno aumento na produção da frutosiltransferase intracelular. Observou-se aumento na produção de frutosiltransferase por Penicillium purpurogenum quando a quantidade de sacarose era restaurada por adição do carboidrato ao meio de cultura durante o período de produção.

Production of a-Glucosidase by Penicillium purpurogenum

Verificou-se que Penicillium purpurogenum foi capaz de produzir a-glucosidase intracelular, com atividade máxima sobre a sacarose. Vários parâmetros culturais para producão da enzima foram otimizados. Verificou-se que a producão máxima da enzima ocorria após 96 h de cultivo a 30º;C. A adicão dos amino-ácidos histidina e cisteína induziram a síntese da enzima até certo ponto. A temperatura e pH ótimos para atividade da enzima foram 50º;C e 5,5, respectivamente. A a-glucosidase de Penicillium purpurogenum foi estável em pH 2,0, o que torna a enzima ideal para uso na indústria de sucos de frutas e vinhos.