Delta-aminolevulinic acid dehydratase (ALAD) activity in blood of Bufo arenarum (Anura)

The aim of the present investigation was to standardize a method for measuring delta-aminolevulinic acid dehydratase (ALAD) activity in circulating red blood cells of adult Bufo arenarum kept in controlled environmental conditions, and to obtain reference basal values suitable for environmental monitoring of lead exposure. The normal ALAD activity for B. arenarum was 131.86 + 14.47 U per liter of red blood cells (n = 38, mean + SEM; interval 72.98 - 263.33). In animals exposed to lead, ALAD activity decreased as lead dose increased.