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Braz. j. microbiol ; 45(1): 279-286, 2014. graf, tab
Article Dans Anglais | LILACS | ID: lil-709463

Résumé

Cellulase production was evaluated in two reference strains (T. reesei Rut-C30 and T. reesei QM9414), two strains isolated from a sugarcane cultivation area (Trichoderma sp. IPT778 and T. harzianum rifai IPT821) and one strain isolated in a program for biodiversity preservation in São Paulo state (Myceliophthora thermophila M77). Solid state cultures were performed using sugarcane bagasse (C), wheat bran (W) and/or soybean bran (S). The highest FPA was 10.6 U/gdm for M77 in SC (10:90) at 80% moisture, which was 4.4 times higher than production in pure W. C was a strong inducer of cellulase production, given that the production level of 6.1 U/gdm in WC (40:60) was 2.5 times higher than in pure W for strain M77; T. reesei Rut-C30 did not respond as strongly with about 1.6-fold surplus production. S advantageously replaced W, as the surplus production on SC (20:80) was 2.3 times relative to WC (20:80) for M77.


Sujets)
Biotechnologie/méthodes , Cellulase/métabolisme , Milieux de culture/composition chimique , Champignons/enzymologie , Champignons/croissance et développement , Fibre alimentaire/métabolisme , Saccharum/métabolisme , Sordariales/enzymologie , Sordariales/croissance et développement , Glycine max/métabolisme , Trichoderma/enzymologie , Trichoderma/croissance et développement
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