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1.
Braz. j. microbiol ; 44(3): 795-798, July-Sept. 2013. tab
Article Dans Anglais | LILACS | ID: lil-699787

Résumé

Although several invasive and noninvasive tests have been developed for the diagnosis of Helicobacter pylori infection, all of the tests have their limitations. We conducted a study to investigate and compare the suitability of rapid urease test (RUT), serology, histopathology and stool antigen tests with polymerase chain reaction (PCR) for detection of H. pylori, and correlate the diagnostic methods with PCR. Eighty nine patients (61 adults, 28 children) referred to the Firoozgar Hospital and Children Medical Center Hospital for diagnostic upper gastrointestinal endoscopy entered to the study and noninvasive tests such as immunoassay for serological antibodies against H. pylori and detection of its antigen in feces were measured. The biopsies were utilized for histological examination, RUT and PCR. The H. pylori statuses were evaluated by the positivity of ureC PCR in biopsy specimens and 53 subjects had H. pylori positive result. Histopathology showed high overall performance in adults and children with sensitivity and specificity 100% and 90%, respectively. Sensitivity, specificity, and accuracy for stool antigen test were 87.8%, 75% and 82%, respectively. Correlation of RUT, serology (IgG), histopathology and stool antigen tests with PCR were 0.82, 0.32, 0.91 and 0.63, respectively. In conclusion, the RUT and histopathology are as accurate as the PCR of biopsy and stool antigen test can consider as appropriate noninvasive test for detection of H. pylori infection.


Sujets)
Adolescent , Adulte , Sujet âgé , Sujet âgé de 80 ans ou plus , Enfant , Humains , Mâle , Adulte d'âge moyen , Jeune adulte , Techniques de laboratoire clinique/méthodes , Tests diagnostiques courants/méthodes , Infections à Helicobacter/diagnostic , Helicobacter pylori/isolement et purification , Antigènes bactériens/analyse , Biopsie , Analyse chimique du sang , ADN bactérien/analyse , ADN bactérien/génétique , Fèces/composition chimique , Muqueuse gastrique/microbiologie , Histocytochimie , Réaction de polymérisation en chaîne , Sensibilité et spécificité , Urease/analyse
3.
Article Dans Anglais | IMSEAR | ID: sea-173915

Résumé

Brucellosis is considered a known widespread zoonotic disease and is endemic in Mediterranean region, like Iran. This study reviewed the clinical manifestations, laboratory findings, and therapeutic regimen in childhood brucellosis in Iran. In this retrospective study, we reviewed hospital-records of 34 consecutive children with a confirmed diagnosis of brucellosis among a total number of 10,864 patients admitted to Children’s Medical Center, Tehran, Iran, between 2002 and 2010. Among the patients diagnosed with brucellosis, 22 (65%) were admitted during spring and summer. Clinical findings of these patients at admission were arthritis, splenomegaly, hepatomegaly, lymphadenopathy, maculopapular skin rashes, and fever. Anaemia (53%) and leukopenia (33%) were the most common findings in the children. Only one patient had presented with leukocytosis. Four children (12%) were thrombocytopenic, and none of patients had pancytopenia. Blood cultures were positive in 5 patients (23%). Only one patient underwent bone-marrow aspiration and had positive culture for Brucella spp. Positive titres were found in 33 cases (97%) in Wright test, 23 cases (96%) in Coombs test, and 16 patients (72.7%) in 2ME (2-Mercaptoethanol) test. In one case, Wright and Coombs test titres were below 1:80 while Brucella spp. were isolated from blood at the same time. It is concluded, prolonged fever with joint involvement and organomegaly may increase possibility of infection with Brucella spp. Appropriate treatment regimen by more tolerable oral drugs, with a duration of at least 8 weeks, is recommended.

4.
Braz. j. microbiol ; 43(2): 766-769, Apr.-June 2012. tab
Article Dans Anglais | LILACS | ID: lil-644494

Résumé

Emerging antimicrobial resistance rates and Extended-spectrum beta-lactamase producing Escherichia coli recovered from urinary tract infections (UTI) is an increasing problem in specific regions, limiting therapeutic options. One hundred E. coli isolates causing UTI in patients with age from 2 months to 12 years admitted at CMC in the period of April 2009 to March 2010 were tested for antibiotic susceptibility using the disk diffusion method. Surprisingly high resistance rates were recorded for E. coli against TMP/SMX (84%), cefalotin (66%), cefuroxime (50%), cefixime (50%) and ceftriaxone (45%). Antimicrobial susceptibility of E. coli isolates was followed by meropenem (98%), amikacin (95%), nitrofurantoin (91%) and gentamicin (68%). Extended spectrum beta-lactamase production, was observed in 32% of community and 42% of nosocomial isolates. The results of this study and numerous observations regarding the increasing resistance to these antibiotics, in several countries, emphasize the need for local population-specific surveillance for guiding empirical therapy for UTI in children.


Sujets)
Humains , Nouveau-né , Adolescent , Techniques et procédures diagnostiques , Prédisposition aux maladies , Résistance microbienne aux médicaments , Infections à Escherichia coli , Escherichia coli/isolement et purification , bêta-Lactamases/analyse , bêta-Lactamases/isolement et purification , Activation enzymatique , Méthodes , Patients , Surveillance des Risques ou des Catastrophes , Urine
5.
Braz. j. infect. dis ; 14(3): 252-255, May-June 2010. ilus, tab
Article Dans Anglais | LILACS | ID: lil-556837

Résumé

BACKGROUND: Fever as a common presenting complaint in pediatric patients can be due to various causes. Differentiating bacterial infection from other causes is important because the prompt use of antibiotics is critical in bacterial infection. Traditional markers of infection such as BT and WBC count may be unspecific and culture may be late or absent. CRP and Procalcitonin (PCT) have been considered to evaluate the evolution of infections and sepsis in patients presenting with SIRS. Neopterin has also been proposed to aid in the diagnosis of bacterial infection. In this study, we compared the value of the serum PCT, neopterin level, and WBC count for predicting bacterial infection and outcome in children with fever. METHODS: 158 pediatric (2-120-month-old) patients suspected to have acute bacterial infection, based on clinical judgment in which other causes of SIRS were ruled out were included in the study. WBC count with differential was determined and PCT and neopterin levels were measured. RESULTS: PCT level was higher in bacterial infection and patients who were complicated or expired. CONCLUSION: Rapid PCT test is superior to neopterin and WBC count for anticipating bacterial infection, especially in ED where prompt decision making is critical. ABBREVIATIONS: BT, body temperature; WBC, white blood cell; PCT, procalcitonin; CRP, C-reactive protein; SIRS, systemic inflammatory response syndrome; ED, emergency department.


Sujets)
Enfant , Enfant d'âge préscolaire , Humains , Nourrisson , Infections bactériennes/diagnostic , Calcitonine/sang , Néoptérine/sang , Précurseurs de protéines/sang , Syndrome de réponse inflammatoire généralisée/diagnostic , Maladie aigüe , Infections bactériennes/sang , Marqueurs biologiques/sang , Test ELISA , Numération des leucocytes , Valeur prédictive des tests , Sensibilité et spécificité , Syndrome de réponse inflammatoire généralisée/sang
6.
Braz. j. infect. dis ; 14(2): 153-157, Mar.-Apr. 2010. tab
Article Dans Anglais | LILACS | ID: lil-548460

Résumé

Appropriate antimicrobial treatment of shigellosis depends on identifying its changing resistance pattern over time. We evaluated 15,255 stool culture submitted from July 2001 to June 2006 to the Laboratory of Children Medical Center Hospital. Specimen culture, bacterial identification, and disk diffusion susceptibility testing were performed according to National Committee for Clinical Laboratory Standards guidelines. From 15,255 stool samples, 682 (4.5 percent) were positive for Shigella species. The most common species of Shigella were S. flexneri (48 percent) and S. sonnei (45 percent); other results were S. dysenteriae (5 percent) and S. boydii (2 percent). The rate of Sensitivity to ceftriaxone (95 percent), ceftizoxime (94 percent), and nalidixic acid (84 percent) were among our isolates. Resistance to co-trimoxazole and ampicillin was 87 percent and 86 percent, respectively. S. flexneri was more multiresistant than other species (47.9 percent). Our isolates are overall most sensitive to ceftriaxone, ceftazidime, and nalidixic acid (> 84 percent). They were most resistant to co-trimoxazole and ampicillin (> 86 percent). Because resistance varies according to specific location, continuous local monitoring of resistance patterns is necessary for the appropriate selection of empirical antimicrobial therapy.


Sujets)
Enfant , Humains , Antibactériens/pharmacologie , Fèces/microbiologie , Shigella/effets des médicaments et des substances chimiques , Tests d'agents antimicrobiens par diffusion à partir de disques , Dysenterie bacillaire/microbiologie , Iran , Shigella/classification , Shigella/isolement et purification
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