Résumé
OBJECTIVE: To investigate the application of NBNA and TIMP in the follow-up of preterm infants,and to as⁃sess the value of them in early diagnosis of abnormal motor development of preterms. METHODS: Databases including Pubmed,Embase and Cochrane Library,China National knowledge internet,and Wanfang data were searched for studies about neuro-behavior or motor assessments with NBNA or TIMP. The methodological quality of the included studies was assessed with COSMIN checklist,then the predictive validity of NBNA and TIMP were compared. RESULTS: Ultimately,16 studies were included,5 of which were about the application of NBNA in follow-up of preterm infants. But many fac⁃tors might pose threat to methodological quality. As for TIMP,11 studies were included,9 of which showed that with a satisfying predictive validity(sensitivity:0.50 to 1.00;specificity:0.68 to 1.00)or moderate correlation(r:0.36 to 0.44)for the neurodevelopmental scores after half a year old. TIMP is helpful in making early diagnosis for neurodevelopmental disability. CONCLUSION: With satisfying predictive validity for long-term neurological development outcomes of the pre⁃term infants,TIMP is considered as an alternative method of assessing functional movements in preterms.
Résumé
<p><b>OBJECTIVE</b>To explore the effect of interfering ADAM10 on proliferation and apoptosis of multiple myeloma MM.1S cells, and its possible mechanism.</p><p><b>METHODS</b>Four pairs of shRNA-coding sequences directed against different sites of ADAM10 mRNA were designed and inserted into lentiviral vector plasimd pLVshRNA-EGFP(2A) Puro for constructing the sh/ADAM10-1, sh/ADAM10-2, sh/ADAM10-3, sh/ADAM10-4 and sh/Con. These plasmids and lentiviral packaging plasmids were co-transfected into the packaging cells 293FT, then the virus particles were collected and the viral titer was assayed after concentration, and these viral particles were transfected to MM.1S cells. The flow cytometry was used to sort GFPcells. Real-time quantitative PCR, and Western blot were used to detect the effect of interfering the ADAM10 gene by lentiviral vector mediated shRNA. The proliferation-inhibition curve was plotted by CCK-8 method, the cell viability and apoptosis were detected by flow cytometry with Annexin V and 7-AAD staining, the transcripts of pro-apoptosis gene BAD, BAK, BIK, anti-apoptotic genes BCL-2, c-Myc and Notch1 target gene Hes-1 were detected by real-time PCR.</p><p><b>RESULTS</b>Lentivirus vector was successfully constructed, that could specifically interfere ADAM10 expression. Interfering ADAM10 gene could inhibit the MM.1S cell proliferation and induce apoptosis. After the interferencing ADAM10 gene the mRNA levels of pro-apoptosis gene BAD, BAK and BIK were increased, and the mRNA levels of anti-apoptotic genes BCL-2 and c-Myc were reduced. Q-PCR results showed that the mRNA level of Notch1 were increased, but that of Hes-1 were reduced.</p><p><b>CONCLUSION</b>Down-regulated ADAM10 expression can significantly inhibit multiple myeloma MM.1S cell proliferation and promote the apotosis. Its mechanism may be related to Notch1 signaling pathways.</p>