Efficient gene editing in a medaka (Oryzias latipes) cell line and embryos by SpCas9/tRNA-gRNA / 浙江大学学报（英文版）（B辑：生物医学和生物技术）

Generation of mutants with clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated protein 9 (Cas9) is commonly carried out in fish species by co-injecting a mixture of Cas9 messenger RNA (mRNA) or protein and transcribed guide RNA (gRNA). However, the appropriate expression system to produce functional gRNAs in fish embryos and cells is rarely present. In this study, we employed a poly-transfer RNA (tRNA)-gRNA (PTG) system driven by cytomegalovirus (CMV) promoter to target the medaka (Oryzias latipes) endogenous gene tyrosinase(tyr) or paired box 6.1 (pax6.1) and illustrated its function in a medaka cell line and embryos. The PTG system was combined with the CRISPR/Cas9 system under high levels of promoter to successfully induce gene editing in medaka. This is a valuable step forward in potential application of the CRISPR/Cas9 system in medaka and other teleosts.

Combine Traditional Chinese and Western Medicine Treatment of Tibial Plateau Fractures Cases of Clinical Observation / 浙江中医药大学学报

[Objective] To study the combination of traditional Chinese and western medicine treatment of tibial plateau fractures cases for clinical observation.[Methods] Randomly select 72 cases of patients in our hospital in 2010-2011, April, make a diagnosis and give treatment with fracture of tibial plateau, divide into two groups, control group 36 cases were treated by routine operation fixed and functional recovery exercise therapy; The team of 36 patients based on treatment with TCM. [Results] The research group patients, excellence rate was significantly higher than the control group, there was significantly statistical differences. Two groups of patients in adverse reactions, through statistical analysis of clinic, research group was better than control group, with statistical difference.[Conclusion] Combining traditional Chinese and western medicine treatment of tibial plateau fracture, the clinical curative effect is distinct, can accurately determine fracture position, select the appropriate clinical treatment, help patients recover at an early date, worthy of clinical application for a long time.