Application of Single Solid State Nanopore / Nanochannel Based on Polymer Membrane and Glass Nanopipette in Analytical Chemistry / 分析化学

Nanopore / nanochannel sensing technique drawing more attention in analytical chemistry due to its unique advantages and the fabricated electrochemical sensors and electrochemical responsive gates have been widely used for more target detection, including single molecule protein and DNA sequencing. Nanopore /nanochannel that used for fabricating electrochemical detection system is mainly divided into biological nanopore and solid state nanopore, and among them, solid state nanopore / nanochannel has a wide range of application due to its inherent properties, such as easy for modification, good mechanical property and stability. Resistive pulse sensing and current-voltage curves (ion current rectification) are two main methods of nanopore / nanochannel sensing technique used for target analysis, so in this review, we introduced the fundamentals and applications of nanopore sensing technique based on the above two methods. In addition, we concluded the application and development of single state nanopore / nanochannel in recent years, and also gave a brief look at the future challenges and prospects in the development of this field.

Relationship between pesticide exposure and adverse pregnancy outcomes among famers: a meta-analysis / 中华劳动卫生职业病杂志

<p><b>OBJECTIVE</b>To analyze the relationship between pesticide exposure and adverse pregnancy outcomes in famers.</p><p><b>METHODS</b>A search was conducted to collect the articles about the relationship between pesticide exposure and adverse pregnancy outcomes published worldwide from 1990 to February 2012. Meta-analysis was performed on the collected articles using RevMan 4.2 software.</p><p><b>RESULTS</b>Twelve articles were collected. Compared with the controls, the pesticide-exposed famers showed a combined odds ratio (OR) for spontaneous abortion of 1.52 (95%CI: 1.04 ∼ 2.21; P = 0.03), a combined OR for premature birth of 1.33 (95%CI: 1.09 ∼ 1.61; P = 0.005), a combined OR for dead fetus of 1.22 (95%CI: 1.16 ∼ 1.29; P < 0.01), a combined OR for stillbirth of 1.90 (95%CI: 0.58 ∼ 6.28; P = 0.29), a combined OR for birth defect of 2.02 (95%CI: 0.84 - 4.69; P = 0.12), a combined OR for low birth weight of 1.62 (95%CI: 0.60 ∼ 4.39; P = 0.34), a combined OR for neonatal death of 2.18 (95%CI: 0.54 ∼ 8.88; P = 0.28), and a combined OR for delayed conception of 1.43 (95%CI: 0.93 ∼ 2.18; P = 0.1). Pesticide exposure increased the risks for spontaneous abortion, premature birth, and dead fetus, but was not significantly associated with stillbirth, birth defect, low birth weight, neonatal death, and delayed conception.</p><p><b>CONCLUSION</b>Pesticide exposure can cause adverse pregnancy outcomes in farmers, increasing the risks of spontaneous abortion, premature birth, and dead fetus.</p>

Delayed protection of HO-1 in the exercise preconditioning from the myocardial relative ischemic reperfusion injury / 中国应用生理学杂志

<p><b>AIM</b>To explore the role of HO-1 in the exercise preconditioning protecting the rat myocardium from the relative myocardial ischemia/reperfusion injury (rI/R).</p><p><b>METHODS</b>40 Wistar rats were divided into 5 groups randomly: control group (CN), relative ischemia/reperfusion group (IR), exercise preconditioning + relative ischemia-reperfusion group (EI), HO-1 revulsant group (HE) and exercise preconditioning + HO-1 inhibitor (EZ) group. We detected the cardiac function parameter--pressure-rate product (PRP), the levels of MDA in coronary effluent and the activity of HO-1.</p><p><b>RESULTS</b>The activities of HO-1 of EI group and HE group were higher significantly than the IR group. EZ group was lower than EI group. There was significant difference between EI group and HE group. The recovery rate of PRP in the 60 min point of reperfusion of EI group was higher significantly than IR group. EZ group was lower than EI group. There was significant difference between HE group and IR group in 30 min point of reperfusion. The levels of MDA in coronary effluent after rI/R of EI group, EZ group and HE group were lower significantly than IR group. There was significant difference between EI group and EZ group.</p><p><b>CONCLUSION</b>EP can activate the HO-1 which can ameliorate the rI/R injury occurred after 24 hours.</p>

The delayed protection of exercise preconditioning from the relative myocardial ischemia-reperfusion injury / 中华劳动卫生职业病杂志

<p><b>OBJECTIVE</b>To explore the delayed protection of exercise preconditioning from the relative myocardial ischemia-reperfusion injury.</p><p><b>METHODS</b>The experiment included the vivo experiment and the vitro experiment, 32 Wistar rats in each experiment were divided into 4 groups randomly: control group (CN), relative ischemia reperfusion group (IR), exercise preconditioning group (EP) and Exercise preconditioning + relative ischemia-reperfusion group (EI). We detected the third loading exercise time, the levels of MDA in serum in vivo experiment and the Cardiac function parameter, the levels of MDA in coronary effluent in vitro experiment.</p><p><b>RESULTS</b>(1) The vivo experiment: The third loading exercise time of EI group [(71.67 +/- 9.00) min] increased significantly compared with that of IR group [(58.67 +/- 4.13) min] (P < 0.05); The levels of MDA in serum of EP group (107.00 +/- 35.99) micromol/L and EI group [(152.23 +/- 29.94) micromol/L] decreased significantly contrasted to IR group (313.20 +/- 43.40 micromol/L) (P < 0.05). (2) The vitro experiment: The PRP (heart rate * left ventricular developed pressure) in reperfusion period of CN group and EP group were stable relatively, while it reached the peak after 30 minutes and almost recovered to the level before ischemia in EI group. The parameter of IR group recovered slightly but was lower significantly than that before ischemia. There was significant difference between the recovery rate of Cardiac function of EI group and that of IR group. The increase of MDA in coronary effluent after Ischemia-reperfusion of EP group (0.34 +/- 0.24 micromol/L) and EI group [(0.41 +/- 0.26) micromol/L] decreased significantly contrasted to that of IR group [(1.27 +/- 0.52) micromol/L] (P < 0.05).</p><p><b>CONCLUSION</b>EP has the obvious delayed protection from the relative myocardial ischemia-reperfusion injury.</p>