Influence of blastocysts morphological score on pregnancy outcomes and neonate′s condition in vitrified-warmed transfer cycles / 实用医学杂志

Objective To estimate the effect of blastocysts morphological score on pregnancy outcomes and neonate′s condition in vitrified-warmed single-blastocyst transfer cycles. Methods A retrospective analysis of 586 cycles of vitrified-warmed single-blastocyst transfer from Mar. 2010 to Feb.2016 was performed and the influ-ence of day of vitrification,inner cell mass(ICM)and trophectoderm(TE)scores on pregnancy outcomes and neo-nate′s condition were observed. Results There were no significant differences in clinical pregnancy rate,birth weight and sex ration of newborn between different vitrification day,ICM score and TE score.The day of vitrifica-tion and ICM score can significantly influence pregnancy loss rate,and were the two primary predictors of pregnan-cy loss rate. Vitrification day,ICM score and TE score exerted significant influence on live birth rate(P < 0.05) and TE score was the primary factor of live birth rate. Conclusions Day 5 vitrified blastocysts with higher quality of ICM and TE can provide high live birth rate and low pregnancy loss rate,but it could not predict the weight and gender of the newborn.

Rapid identification of cortex dictamni pieces and its counterfeit alangium Chinense by spectral imaging method / 中国中药杂志

<p><b>OBJECTIVE</b>The spectral imaging method was used to quickly identify the Cortex Dictamni pieces and its counterfeit alangium Chinese, which is a potential method would be applied to control the quality of the Cortex Dictamni pieces.</p><p><b>METHOD</b>Standard sample, 5 cortex dictamni samples and its counterfeit alangium Chinese of different sources were tested by the liquid crystal imaging instrument. The spectrum resolution was 5 nm, the spectral range was from 405 nm to 680 nm, and the spatial resolution was 50 lp x mm(-1). The characteristic spectrum curves were picked up from spectral cube and principal analysis method was used to analyze the results.</p><p><b>RESULT</b>The identification results by the spectral imaging method accorded well with the results by the traditional biology and chemistry analysis method.</p><p><b>CONCLUSION</b>The spectral imaging analysis method can be used to identify the cortex dictamni pieces and its counterfeit alangium Chinense. The testing course is convenient, quick and noninvasive.</p>

CONTROLLED OVARIAN HYPERRSTIMULATION SIGNIFICANTLY DECREASED THE EXPRESSION OF AQUAPORIN-3 IN MOUSE OOCYTES USING SEMI-QUANTITATIVE REAL-TIME PCR ANALYSIS / 解剖学报

Objective To study the effect of controlled ovarian Hyperstimulation(COH)on expression of AQP3 mRNA in mouse oocytes at metaphase Ⅱ. Methods Twenty female mice(6-7 weeks) were randomly allocated into 2 groups, mice in COH group were superovulated by intraperitoneal injection of 7.5 IU pregnant mare's serum gonadotropin(PMSG) followed by 5 IU human chorionic gonadotropin(HCG) after 46-48?h. Nothing was given to mice in control group and the estrus cycle was observed at 9?am everyday. 12-16?h following hCG injection (COH group) or at 8?am next day after the estrus (control group), mice were killed by cervical dislocation. The oviducts were excised.Cumulus masses were recovered from the dilated ampullae under a dissecting microscope,digested granulosa cells using hyaluronidase. Semi-quantitative real-time PCR of AQP3 mRNA in mouse MⅡoocytes was investigated with ?-actin as the internal control. Results Oocytes swelling assay showed that AQP3 mRNA expressed in mouse MⅡ oocytes. Using semi-quantitative real-time PCR, the expression of AQP3 mRNA was significantly decreased(P