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Article de Chinois | WPRIM | ID: wpr-252705

RÉSUMÉ

<p><b>AIM</b>The mechanisms of cytokines in regulating oocyte maturation is still little known. The present study attempt to investigate whether the protooncogene of c-erbB2, c-myb are involved in introducing of cytokines to regulate oocyte maturation.</p><p><b>METHODS</b>This research used mouse GV stage oocyte culture model in vitro and RT-PCR, Western blotting method to explore the effect of EGF, TNFalpha, ET-1 and NO on oocyte maturation; to analyze the c-erbB2 mRNA and c-myb mRNA expression and the phosphorylation of MAPK and cyclinB1 expression in oocytes affected by above cytokines.</p><p><b>RESULTS</b>EGF(10 microg/L) stimulated meiosis of oocytes significantly, the level of c-erbB2 mRNA, c-myb mRNA were increased, and promoted the phosphorylation of MAPK and cyclinB1 expression; TNFalpha (1 microg/L) and ET-1 ((10(-1) mol/L) had the results to EGF. Low dose of SNP (10(-5)mol/L) had no effect on oocyte maturation, but could significantly reverse the suppression of dbcAMP on oocyte maturation.</p><p><b>CONCLUSION</b>c-erbB2 and c-myb were involved in introducing of cytokines to regulate oocyte maturation, might be the middle link in connection of the cytokines with MAPK and MPF in regulation oocyte maturation.</p>


Sujet(s)
Animaux , Femelle , Souris , Cellules cultivées , Cytokines , Physiologie , Facteur de croissance épidermique , Physiologie , Protéines et peptides de signalisation intercellulaire , Physiologie , Facteur de promotion de la maturation , Génétique , Métabolisme , Mitogen-Activated Protein Kinases , Métabolisme , Ovocytes , Biologie cellulaire , Physiologie , Ovogenèse , Physiologie , Protéines proto-oncogènes c-myb , Génétique , Métabolisme , ARN messager , Génétique , Métabolisme , Récepteur ErbB-2 , Génétique , Métabolisme , Facteur de nécrose tumorale alpha , Physiologie
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