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Journal of Veterinary Science ; : 399-407, 2014.
Article Dans Anglais | WPRIM | ID: wpr-194858

Résumé

A recombinant replication-defective adenovirus expressing the major epitopes of porcine circovirus-2 (PCV-2) capsid protein (rAd/Cap/518) was previously constructed and shown to induce mucosal immunity in mice following intranasal delivery. In the present study, immune responses induced by intranasal immunization with a combination of rAd/Cap/518 and cytosine-phosphate-guanosine oligodeoxynucleotides (CpG ODN) were evaluated in mice. The levels of PCV-2-specific IgG in serum and IgA in saliva, lung, and intestinal fluids were significantly higher in the group immunized with rAd/Cap/518 and CpG ODN than animals immunized with rAd/Cap/518 alone. The frequencies of IL-2-secreting CD4+ T cells and IFN-gamma-producing CD8+ T cells were significantly higher in the combined immunization group than mice immunized with rAd/Cap/518 alone. The frequencies of CD3+, CD3+CD4+CD8-, and CD3+CD4-CD8+ T cells in the combined immunization group were similar to that treated with CpG ODN alone, but significantly higher than mice that did not receive CpG ODN. PCV-2 load after challenge in the combined immunization group was significantly lower than that in the phosphate-buffered saline placebo group and approximately 7-fold lower in the group treated with CpG ODN alone. These results indicate that rAd/Cap/518 combined with CpG ODN can enhance systemic and local mucosal immunity in mice, and represent a promising synergetic mucosal vaccine against PCV-2.


Sujets)
Animaux , Femelle , Souris , Adenoviridae/génétique , Administration par voie nasale , Protéines de capside/génétique , Infections à Circoviridae/immunologie , Circovirus/génétique , Épitopes/génétique , Immunité muqueuse/immunologie , Immunoglobuline A/sang , Immunoglobuline G/sang , Souris de lignée BALB C , Oligodésoxyribonucléotides/génétique , Vaccins synthétiques/génétique , Vaccins antiviraux/administration et posologie
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