Expression of miRNA-22 in ovarian cancer and effect of miRNA-22 over-expression on SKOV-3 ovarian cancer cell proliferation, migration and in-vasion / 中国病理生理杂志

AIM: To examine the expression of miRNA-22 in the ovarian tissues and the effect of miRNA-22 over-expression on the proliferation, migration and invasion in SKOV-3 cells.METHODS: The expression levels of miRNA-22 in different ovarian tissues and SKOV-3 cells were determined by qPCR .miRNA-22 was over-expressed by trans-fection of miRNA-22 mimic.The cell viability was examined by CCK-8 assay.The cell migration was measured by wound healing test .The cell invasion was analyzed by Transwell assay .The protein expression levels of VEGF and P 53 were deter-mined by Western blot .RESULTS: Compared with the normal ovarian tissue , the expression level of miRNA-22 was remarkably decreased in the ovarian tumor tissues .After transfection with miRNA-22 mimic, the expression level of miRNA-22 in the SKOV-3 cells was significantly increased , while the cell viability , migration and invasion were obviously decreased .Moreover , the protein expression of VEGF and P 53 was dramatically inhibited after over-expression of miRNA-22.CONCLUSION:The decreased miRNA-22 expression may be correlated with the development of ovarian can-cer.Over-expression of miRNA-22 decreases the cell viability , migration and invasion by reducing the protein expression of VEGF and P53.

Effect of Glutamine's nutrition support on the postoperative nutrition and immune function in malignant tumor of gynecology patients

This paper is mainly to study the influence of Glutamine's reinforcing role in enteral nutrition on postoperative nutrition and immune function of patients with gynecologic malignant tumor

The adopted method is through selecting 60 patients with gynecological malignant tumor, they are randomly divided into groups of enteral immune nutrition [BIN group] and conventional enteral nutrition [EN group], which are given enteral nutrition of equal nitrogen and heat respectively in postoperative 24 hours, the total period of nutritional support are 7 days

The research results represent that nutrition and immunity index in both groups on the first day after operation is lower than the day before operation, and all indicators have picked up on the ninth day after operation