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1.
Iranian Journal of Veterinary Research. 2013; 14 (3): 237-240
Dans Anglais | IMEMR | ID: emr-130802

Résumé

This study was conducted to reveal the estrogenic effects of bisphenol A and o, p'-DDT on quail embryos. Thirteen fertilized eggs were used as control [injected with 20 microl corn oil], 15 eggs were injected with estradiol 17beta [0.04 mg dissolved in 20 microl corn oil], 20 eggs were injected with BPA [2 mg dissolved in 20 microl corn oil] and 20 eggs were injected with o, p'-DDT [2 mg dissolved in 20 microl corn oil] at day 13 of incubation. Two days later the livers of the embryos were collected. The DNA was extracted from the liver for molecular sexing, while total RNA was extracted for vitellogenin II [VTGII] mRNA expression in embryos. In female embryos, BPA and o, p'-DDT induced variable levels of VTGII mRNA expression, while in male embryos, o, p'-DDT induced a slightly VTGII mRNA expression. In contrast, there was no expression of VTGII after BPA injection. In conclusion, the estrogenicity of BPA was lower than o, p'-DDT and both of them were lower than the estradiol 17beta


Sujets)
Animaux , Phénols , DDT , ARN messager , Vitellogénines , Foie , Caille , Expression des gènes , Structures de l'embryon
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