Résumé
Application of quantitative real time PCR has evolved as a sensitive, specific, and rapid method for the detection of Toxoplasma gondii [T. gondii]. The present study aimed to evaluate the efficacy of real time PCR method, using B1 gene, for the diagnosis of toxoplasmosis in the experimentally infected rats. Parasites were cultured in peritoneal cavity of mice and then the DNA was extracted in tachyzoite stage. The B1 gene of T. gondii was amplified by PCR and detected by real time PCR method based on the molecular beacon probe. Finally, real time PCR was evaluated for the quantization of T. gondii in the blood of the experimentally infected rats. The B1 gene of T. gondii which was successfully amplified by PCR yielded an amplicon with an approximate length of 116 bp. Using this gene was evaluated highly appropriate for the quantization of T. gondii by real time PCR method. Application of real time PCR method is shown to be highly efficient in terms of sensitivity and rapidity for the detection of B1 gene as well as the quantization of T. gondii in blood of rat
Résumé
Groundwater treatment by nano particles has received increasing interest in recent years. Chromium is a commonly identified contaminant in soils and groundwater. Zero-valent iron, as a natural reduction agent can be used in controlling of contaminated sites. The aim of this research is investigation of hexavalent chromium removal from aqueous solutions by using of iron nano particles the effective parameters. In this research the synthesized of the iron nano particles has performed by addition of NaBH4 to FeCl3 6H2O solution and Cr[VI] reduction efficiency in Batch system was studied. Also the impact of the important field parameters including pH, initial chromium concentration, nano zero valent iron concentration and retention time were investigated. The results of this research showed that synthesized particles were in nano scale. In pH=3, chromium inlet concentration of 10 mg L-1, nano zero valent iron concentration 0.5 g L-1 and 2 minute retention time, 100% of Cr[VI] was removed. The concentration of nano zero valent iron had significant effect on the reduction of Cr[VI]. The reaction occurred in a wide range of pH value and the reaction efficiency increased significantly with decreasing initial pH. The significant removal efficiency, high rate of process and short reaction time were showed that iron nano particles have significant potential in removal ofCr[VI] from contaminated water