new more simpler and rapid technique for extracting a genomic DNA from mycobacterial strains

A rapid simple method for extraction of the DNA from mycobacteria is described. The extraction of DNA from mycobacteria, simply depends upon the lysis of cell wall, using sodium hydroxide, sodium dodecyl sulfate and sodium chloride. The resulting DNA has been checked by other tests such as polymerase chain reaction [PCR] and the results indicated that the extracted DNA works well with these tests

Delayed hypersensitivity and humoral immune response in steers experimentally infected with different strains of mycobacteria

Four steers were inoculated with M. bovis, M. tuberculosis, M. kansasii and M. fortuitum respectively. A fifth steer was left as an uninoculated control. Results of tuberculin test indicated that reaction to homologous tuberculin was more specific than heterologous tuberculin. Non specific reaction to bovine and human PPD tuberculins was developed in steers injected with strains other than M. bovis. Electrophretic analysis revealed significant increase in total globulins in the serum of the steer experimentally infected with M. bovis in comparison with the control steer. Meanwhile a slight rise in total globulins was noted in M. tuberculosis infected steer but it returned to normal level at the end of the experiment. A non significant change in total globulins was noticed in the sera of the other inoculated steers