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Braz. j. microbiol ; 45(2): 551-557, Apr.-June 2014. ilus, graf
Article Dans Anglais | LILACS | ID: lil-723120

Résumé

The entomopathogenic fungus Beauveria bassiana (Balsamo 1835) Vuillemin is an effective alternative control agent against some agricultural pests and biological vectors of important diseases such as Chagas disease. In this work we studied an isolate of Beauveria bassiana from of the town of San Antonio Rayón, Puebla, Mexico and its entomopathogenic effects on Meccus pallidipennis (Stal 1872). Phylogenetic analysis using molecular comparison of the ITS and EF1α genes, showed that the resulting cladogram places the BUAP 04 strain with a relationship closer to the AFAO 9-6 strain, within the diversity of the B. bassiana sensu lato group. Although there was the possibility that BUAP 04 strain was a direct descendant of strains used in campaigns of biologic control, molecular study allowed us to recognize that it was a different fungus due to numerous inserts. A strain isolated from a T. dimiata was evaluated for pathogenicity against another triatoma (Meccus pallidipennis) species obtaining an LC50 of 4.16 x 10(6) spores/mL, confirming that the BUAP 04 strain is virulent for M. pallidipennis and could be a good prospect for formulations to control M. pallidipennis.


Sujets)
Animaux , Beauveria/croissance et développement , Triatoma/microbiologie , Triatoma/physiologie , Beauveria/classification , Beauveria/génétique , Beauveria/isolement et purification , Analyse de regroupements , ADN fongique/composition chimique , ADN fongique/génétique , ADN des helminthes/composition chimique , ADN des helminthes/génétique , Espaceur de l'ADN ribosomique/composition chimique , Espaceur de l'ADN ribosomique/génétique , Mexique , Données de séquences moléculaires , Phylogenèse , Facteur-1 d'élongation de la chaîne peptidique/génétique , Lutte biologique contre les nuisibles/méthodes , Analyse de séquence d'ADN , Analyse de survie , Virulence
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