modified trehalose-mannitol-phosphatase agar for differentiation of staphylococcus epidermidis and staphylococcus saprophytics from other coagulase-negative staphylococci

Trehalose-mannitol agar was used instead of trehalose-mannitol phosphatase agar for differentiating of staphylococcus epidermidis and Staphylococcus saprophyticus from other coagulase negative staphylococci. Staphylococcus epidermidis showed a sensitivity of 100%, specificity of 89.6% and a positive predictive value of 95.7%. Addition of 5mu g novobiocin disk to the medium differentiates staphylococcus saprophyticus with a sensitivity of 100%, specificity of 97% and a positive predictive value of 86.2%. Trehalose-mannitol- agar is considered as a simple and less expensive medium that yields reliable results

Haemophilus Influenza in acute otitis media among Jeddah citizens, Saudi Arabia

The occurrence of Haemophilus influenzae in acute otitis media of different age groups was studied. Haemophilus influenzae was isolated from 18.7% of all cases with a significant frequency among children 2-5 years old [26.7%]. B-lactamase producing strains were present in 17.2% of all strains without significant difference among different age groups. Type b strains were detected in 1.2% of all cases and from 6.9% of the isolated strains