Résumé
The presence of HBV-DNA in the patient's serum without detectable HBV surface antigen [HBsAg.] called occult infection. Detection of occult HBV infection in chronic hepatitis C virus patients was investigated by using qualitative PCR. Co-infection with occult HBV in chronic HCV patients increases the risk for progression to hepatocellular carcinoma [HCC].Detection of CD45-CD90+ as a biomarker in HCC patients by flow cytometry. We searched for serum HBV DNA in 30 patients with histologically verified HCV-related chronic liver disease, in addition to 10 healthy control subjects collected at National Liver Institute in Shebin El-Kom, Monofiya University, Egypt from January, 2010 to October, 2010. Off 40 patients, the sera of 9 [15.0%] were positive for HBV DNA by the different PCR assays, documenting an occult HBV infection. It found that 5 patient samples are positive for HBV DNA [Surface gene] [12.5%] of total 40 patient samples, also 3 patient samples are positive for HBV DNA [X gene] [7.5%] of total 40 patient samples, and only one patient sample was positive HBV DNA [core gene] [2.5%] of total 40 patient samples. Only two samples from the nine positive samples were positive for both Xgene and Surface gene. In conclusion these data suggest that occult HBV infection may have clinical significance in chronic hepatitis C patients
Résumé
The involvement of CMV in hepatocellular carcinoma [HCC] is not totally defined till now. The aim of this work is to study the prevalence of CMV in HCC patients and its correlation to hepatitis B virus [HBV] and hepatitis C virus [HCV] related HCC carcinogenesis. Forty hepatic disease patients were involved 10 [25%] of them were Not suffering from HCC and 30 with HCC [75%] and its complications. Different investigations have been measured in serum of all patients concerned to liver complications. These measurements were HCV RNA and HBV DNA both using Real- Time PCR technique. CMV, AFP and ANA using ELISA technique. Finally SGOT and SGPT using conventional clinical analysis techniques. Concerning HCC +ve patients 73.4% of them were CMV -ve and 26.6% were CMV +ve, while 16.7% of them were HCV -ve and 83.3% were HCV +ve and 50% of them were HBV -ve and HBV +ve. Concerning HCC -ve patients 80% of them were CMV -ve and 20% were CMV +ve, while all were HCV -ve and HBV -ve. High AFP concentration was observed in 93.4% of HCC +ve patients. In conclusion both HBV and HCV and AFP were found to be highly specific risk factors in HCC patients [P< 0.0001], while CMV viral activities were not a risk factor to HCC patient [P< 0.673]
Résumé
Background HCV is a leading cause of chronic liver diseases, cirrhosis and hepatocellular carcinoma. Liver fibrosis and the end-stage of liver fibrosis, 'Cirrhosis, represent the final common pathway of virtually all chronic liver diseases. During this process different biochemical markers associated with connective tissue turnover are released into the blood for example increased level of procollagen III N-terminal [PIII-NP], decreased serum level of matrix metalloproteinase [MMP- I], increased levels of '7S fragment of type IV collagen, hyaluronic acid, gelatinase A, and tissue inhibitor metalloproteinases
Methods This study was carried out on 50 patients with evidence of chronic hepatitis C, they were [42] males and [8] females. All cases were selected from Out patient clinic of the Hepatology unit of Research Institute for Tropical Medicine..The patients were divided according to the stage offlbrosis into 5 groups from FO to F4 according to metavir stage.serum. MMP-1/] MMP-2 and HA levels determined using enzyme-linked immunosorbent assay technique
Results Our retrospective study determines serum level of Metalloproteinase -l [MMP-l], Metalloproteinase -2 [ MMP-2], and Hyaluronic acid [HA] as non invasive markers of liver fibrosis in chronic hepatitis C and to correlate their serum levels with the stage of flbrosis assessed by histopathological staging of liver biopsy. HA level increased significantly with progression offlbrosis whereas Serum level of MMP-1 and MMP-2 had no statistical significant change with progressive fibrosis
Conclusions Serum level of HA can be used as an independent predictor of significant fibrosis, while other studied markers are dependent predictors of significant fibrosis
Résumé
Background EBV was the first human virus directly implicated in carcinogenesis. Since its discovery it has been considered as a major player in the development of a wide range of cancers. Recent studies have concluded EBV itself or infected cell clones might promote replication of the HCV
EBNAl is responsible for supporting HCV replication, suggesting that EBV may be involved in the development of hepatocellular carcinoma [HCC] The detection of EBV gene products in HCC additionally supports this assumption
Methods Methods This study was carried out on cases divided into three groups:-Group I included 15 very low cone, of EBV [IgG] with both negative HCC and HCV RNA patients, Group 2 included 15 moderate cone, of EBV [IgG] with positive HCC and negative HCV RNA patients and Group 3 included 15 high cone, of EBV [IgG] with both positive HCC and HCV RNA patients Reverse transcription PCR [RT-PCR] was performed to detect HCV RNA
Immunohistochemistry was performed to detect EBV[IgG].. The positive ratios were compared between HCC group and control group
Results Our retrospective study determines serum level of hepatitis C, Epstien - Barr [IgG] and Alpha feto protein and to correlate their serum levels with each other in the patient of hepatocellular carcinoma assessed by histopathological staging of liver biopsy. HCV level increased significantly with progression of Serum level ofEBV[IgG] and AFP had no statistical significant change with EBV[IgG]
Conclusion: The existence of EBV infection in HCC tissues suggests that EBV may be involved in the hepatocellular carcinogenesis in Egypt. HCV infection may be a major cause of HCC. There is correlation between EBV and HCV in the development of HCC. EBV enhancement the replication of HCV