HPV 16 DNA sequences in different grades of cervical lesions.

A simple rapid method for isolation of genomic DNA from cervical scrape cells has been standardized. Sufficient amount of good quality genomic DNA was isolated from each cervical scrape specimen. Total 120 cervical scrape specimens from wide spectrum of cervical lesions were analysed for the presence of HPV 16 DNA by Southern hybridization using 32P labelled HPV 16 DNA as probe under stringent conditions. Viral DNA sequences were detected in 78 of the specimens. About 36% (5 out of 14) of normal cervical smears and 59% (46 out of 78) of inflammatory cervical smears had the viral DNA sequences. Except a lone sample, the rest 27 samples of different grades of dysplasia specimens and squamous cell carcinoma samples had viral DNA sequences. Due to development of sensitive Southern hybridization method, the presence of HPV 16 DNA could be detected in high percentage of specimens belonging to the inflammatory cervical specimens.

Superhelical density of goat mitochondrial DNA: fluorimetric studies.

EtBr binding to DNA has been used to determine the superhelical density of Goat mtDNA. The differential binding of closed circular and nicked circular DNA is estimated by simple fluorescence measurements, which is a function of state of DNA supercoiling. The method is simple and quick over the cesium chloride density gradient centrifugation studies.

Physical microenvironment of platelet membrane in acute myocardial infarction.

Patients with acute myocardial infarction have more reactive platelets than those from normal population. These pathological platelets had more viscous plasma membrane, as inferred from fluorescence polarisation studies, and a lower fusion activation energy (delta E) of the membrane, reflecting a higher degree of order within lipid-lipid interactions.