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1.
Genet. mol. res. (Online) ; 5(2): 323-332, 2006. ilus, graf, tab
Article Dans Anglais | LILACS | ID: lil-442566

Résumé

An insertion mutation within exon 12 of the factor XI gene has been described in Holstein cattle. This has opened the prospect for large-scale screening of cattle using the polymerase chain reaction (PCR) technique for the rapid identification of heterozygous animals. To facilitate such a screening process, the mutant and normal alleles of factor XI gene, represented by 244- and 320-bp PCR amplified fragments, were individually cloned in Escherichia coli using a multicopy plasmid cloning vehicle to generate pFXI-N and pFXI-M, respectively. The authenticity of the inserts was confirmed by nucleotide sequencing. A nested PCR method was developed, by which PCR amplicons generated from primers with annealing sites on the recombinant plasmids and by flanking the insert were used as templates for amplification of the diagnostic products using factor XI gene-specific primers. An equimolar mixture of both PCR amplicons, originating from pFXI-N and pFXI-M, constituted the carrier control while the individual amplicons were the affected and normal controls. The controls were used as references for in-gel comparison to screen a population of 307 cattle and 259 water buffaloes; the frequency of the mutant allele was found to be 0. No DNA size standards were required in this study. The simulated control DNA samples representing normal, carrier and affected cattle have the potential to help in large-scale screening of a cattle population for individuals that are carriers or affected by factor XI deficiency.


Sujets)
Animaux , Bovins , Analyse de séquence d'ADN/médecine vétérinaire , Déficit en facteur XI/médecine vétérinaire , Dépistage des porteurs génétiques/méthodes , Maladies des bovins/génétique , Allèles , Buffles , Données de séquences moléculaires , Déficit en facteur XI/génétique , Génotype , Réaction de polymérisation en chaîne/médecine vétérinaire , Séquence nucléotidique
2.
Indian J Physiol Pharmacol ; 1975 Oct-Dec; 19(4): 221-3
Article Dans Anglais | IMSEAR | ID: sea-108829

Résumé

Urinary excretion of fructose during three hours following the ingestion of 100 g of hydrolysed sucrose (in 200 ml) was studied in 85 normal men. This solution was better tolerated than a solution of 50 g of pure fructose and gave higher urinary excretion of fructose than with 100 g of unhydrolysed sucrose. The mean fructose excretion was 54.0 mg (S.D. 40.6 MG). The majority of subjects (63) excreted between 1 and 75 mg and the highest value was 187mg. This suggests a relatively inexpensive method for assessing the existence of porta-systemic shunts beyond normal.


Sujets)
Adolescent , Adulte , Circulation collatérale , Fructose/diagnostic , Humains , Hydrolyse , Mâle , Adulte d'âge moyen , Système porte/physiologie , Saccharose/diagnostic
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