RÉSUMÉ
This study was performed on 57 NHL cases [31 m and 26 f] with different stages, randomly selected from Medical Oncology Department, Minoufiya University hospitals. All patients were subjected to peripheral blood collection, bone marrow aspiration and biopsy to detect IgH and TCR gene rearrangement using PCR technique. Also all samples were examined for morphological changes by H and E stain, immunohistochemistry using CD20, CD 45R and LCA antibodies; The morphological examination showed 6/57 positive results [10.5%] in the bone marrow aspiration, 17/49 [34.7%] in biopsy, 20/49 [40.8%] in combined aspiration, biopsy and the immunohistochemistry stain. By using PCR method, the IgH gene rearrangement was positive in 32/52 [61.5%] using bone marrow aspiration; while 9/24 [37.5%] in peripheral blood and 35/54 [64.8%] in combined bone marrow and peripheral blood using FR-llla, FR-lla, FR l-c, Vu-Ju and their combination. The TCR-y gene rearrangement detects clonality in 9/52 [17.3%] using bone marrow aspiration, while 11/54 [25.9%] in peripheral blood and 14/54 [25.9%] in combined bone marrow aspiration and peripheral blood examinations