Résumé
BACKGROUND: Current study has been designed to evaluate the chemical composition of essential and fixed oils from stem and leaves of Perovskia abrotanoides and antioxidant and antimicrobial activities of these oils. RESULTS: GC-MS analysis of essential oil identified 19 compounds with (E)-9-dodecenal being the major component in stem and hexadecanoic acid in leaves. In contrast, GC-MS analysis of fixed oil showed 40 constituents with α-amyrin the major component in stem and α-copaene in leaves. The antioxidant activity showed the highest value of 76.7% in essential oil from leaves in comparison with fixed oil from stem (45.9%) through inhibition of peroxidation in linoleic acid system. The antimicrobial assay tested on different microorganisms (e.g. E. coli, S. aureus, B. cereus, Nitrospira, S. epidermis, A. niger, A. flavus and C. albicans) showed the higher inhibition zone at essential oil from leaves (15.2 mm on B. cereus) as compared to fixed oil from stem (8.34 mm onS. aureus) and leaves (11.2 mm on S. aureus). CONCLUSIONS: The present study revealed the fact that essential oil analyzed from Perovskia abrotanoides stem and leaves could be a promising source of natural products with potential antioxidant and antimicrobial activities, as compared to fixed oil.
Sujets)
Anti-infectieux/composition chimique , Antioxydants/composition chimique , Lamiaceae/composition chimique , Feuilles de plante/composition chimique , Huiles végétales/pharmacologie , Tiges de plante/composition chimique , Alcanes/analyse , Alcanes/pharmacologie , Anti-infectieux/pharmacologie , Antioxydants/pharmacologie , Aspergillus/effets des médicaments et des substances chimiques , Bacillus cereus/effets des médicaments et des substances chimiques , Candida albicans/effets des médicaments et des substances chimiques , Tests d'agents antimicrobiens par diffusion à partir de disques , Escherichia coli/effets des médicaments et des substances chimiques , Chromatographie gazeuse-spectrométrie de masse , Peroxydation lipidique/effets des médicaments et des substances chimiques , Tests de sensibilité microbienne , Éthers méthyliques/analyse , Éthers méthyliques/pharmacologie , Huile essentielle/composition chimique , Acide oléanolique/analyse , Acide oléanolique/analogues et dérivés , Acide oléanolique/pharmacologie , Acide palmitique/analyse , Acide palmitique/pharmacologie , Triterpènes pentacycliques/analyse , Triterpènes pentacycliques/pharmacologie , Huiles végétales/composition chimique , Réducteurs/analyse , Sesquiterpènes/analyse , Sesquiterpènes/pharmacologie , Staphylococcus/effets des médicaments et des substances chimiques , Acides stéariques/analyse , Acides stéariques/pharmacologieRésumé
Bambusa arundinacea locally known as Bans or bamboo, a perennial fastest growing plant. This plant is medicinally much famous. The biological activities of this plant were carried out by using standard procedures. The aim of this work was to investigate the in vitro antimicrobial and haemolytic activities of Bambusa arundinaceae leaves. The GC-MS analysis revealed that the major components determined in n-hexane extract were as n-Nonane (17.14%), 2,2,3-Trimethylbutane (5.85%), Tridecane (5.42%), and 3,4,5,6-Tetramethyloctane (5.32%). The disc diffusion and modified resazurin microtitre-plate assays were used to evaluate the inhibition zones (IZ) and minimum inhibitory concentration (MIC) of B. arundinaceae leaves extracts. The plant extracts showed considerable antimicrobial activity. The heamolytic activity against human blood erythrocytes (RBCs) was studied and the % lysis of RBCs was found to be in the range of 1.03 to 4.81. The results of the present study confirmed the considerable antimicrobial and haemolytic activities of the plant Bambusa arundinaceae.