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Braz. j. microbiol ; 46(1): 237-249, 05/2015. tab, graf
Article Dans Anglais | LILACS | ID: lil-748255

Résumé

This study aimed to characterize the safety and technological properties of Enterococcus faecium strains isolated from Brazilian Coalho cheeses. High levels of co-aggregation were observed between Enterococcus faecium strains EM485 and EM925 and both Escherichia coli and Clostridium perfringens. Both strains presented low levels of hydrophobicity. E. faecium EM485 and EM925 were both able to grow in the presence of 0.5% of the sodium salts of taurocholic acid (TC), taurodeoxycholic acid (TDC), glycocholic acid (GC), and glycodeoxycholic acid (GDC), although they showed the ability to deconjugate only GDC and TDC. Both strains showed good survival when exposed to conditions simulating the gastro intestinal tract (GIT). When tested for the presence of virulence genes, only tyrosine decarboxylase and vancomycin B generated positive PCR results.


Sujets)
Fromage/microbiologie , Enterococcus faecium/isolement et purification , Enterococcus faecium/physiologie , Sécurité des aliments , Manipulation des aliments/méthodes , Adhérence bactérienne , Brésil , Phénomènes chimiques , Acides choliques/métabolisme , Acides choliques/toxicité , Clostridium perfringens/composition chimique , Clostridium perfringens/physiologie , Enterococcus faecium/composition chimique , Escherichia coli/composition chimique , Escherichia coli/physiologie , Tube digestif/composition chimique , Interactions hydrophobes et hydrophiles , Inactivation métabolique , Viabilité microbienne/effets des médicaments et des substances chimiques , Réaction de polymérisation en chaîne , Facteurs de virulence/analyse , Facteurs de virulence/génétique
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