1.
Mem. Inst. Oswaldo Cruz
;
89(Suppl.2): 37-41, 1994.
Article
Dans Anglais
| LILACS
| ID: lil-319954
Résumé
The 21kD ookinete antigen of Plasmodium berghei (Pbs 21) has been shown to elicit an effective and long lasting transmission blocking immune response in mice. Having cloned and sequenced this antigen (Paton et al. 1993) the sequence was compared to the genes of the same family previously identified in P. falciparum, P. gallinaceum (Kaslow et al. 1989) and P. reichenowi (Lal et al. 1990). Four conserved areas were identified in this comparison, to which degenerate oligonucleotides were designed. PCR amplification and screening of genomic libraries was then carried out using these oligonucleotides. The P. yoelii gene was successfully cloned and a number of novel P. vivax genes identified but the P. vivax homologue of Pbs21 remains elusive.
Sujets)
Animaux , Souris , Gènes de protozoaire/génétique , Plasmodium , Analyse de séquence d'ADN/méthodes , Séquence nucléotidique , Cartographie chromosomique , Banque de gènes , Code génétique , Données de séquences moléculaires , Plasmodium berghei , Plasmodium vivax , Plasmodium yoelii , Réaction de polymérisation en chaîne
2.
Acta odontol. latinoam
;
1(1): 15-24, 1984. ilus, tab
Article
Dans Anglais
| LILACS
| ID: lil-55593