Résumé
A total of 11 Vibrio cholerae isolates from 1996-1998 outbreaks in Malaysia and 4 V. alginolyticus were analyzed. Isolates were characterized by polymerase chain reaction (PCR) and Southern hybridization for the presence of the gene encoding zonula occludens toxin (zot). Screening of zot gene by PCR revealed the presence of this gene in V. cholerae and V. alginolyticus. The zot gene from one V. cholerae Ogawa isolate that was cloned in a pCR 2.1 TOPO vector was sequenced. The sequences obtained were 99% homologous to the zot gene sequence from the Gene Bank.
Sujets)
Séquence nucléotidique , Technique de Southern , Toxine cholérique/génétique , Clonage moléculaire , ADN bactérien , Humains , Données de séquences moléculaires , Réaction de polymérisation en chaîne , Similitude de séquences d'acides nucléiques , Spécificité d'espèce , Vibrio/génétique , Vibrio cholerae/génétiqueRésumé
Frogs caught from two States (Selangor and Langkawi) in Malaysia were examined for spargana of Spirometra sp. Infected frogs usually show no marks of infection but some had swelling and bleeding at the infection site. The size and weight of the infected frogs did not correlate with the infection status. The infection status in relation to human health is discussed.