Résumé
<p><b>OBJECTIVE</b>To establish homozygous transgenic mouse strain expressing human tau isoform with P301L mutation.</p><p><b>METHODS</b>Five transgenic mice expressing human tau isoform with P301L mutation were obtained by microinjection into male nuclei. Homozygote and hemizygote were identified by PCR and real-time fluorescent quantitative PCR.</p><p><b>RESULTS</b>Ninety five homozygous transgenic mice were selected, and the results indicated that homozygous transgenic mice were superior to hemizygote in simulating the changes of biological characteristics.</p><p><b>CONCLUSION</b>Exogenous gene tau is able to stably transmit to next generation and the combination of SYBR Green real-time fluorescent quantitative PCR with the traditional mating is a fast, reliable and economical way to screen homozygous and hemizygous transgenic mice.</p>