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Southeast Asian J Trop Med Public Health ; 2005 May; 36(3): 609-15
Article Dans Anglais | IMSEAR | ID: sea-33435

Résumé

Anopheles minimus Theobald is one of the major vectors of malaria throughout the Oriental Region, and it's complex consists of at least 2 sibling species (A and C) in Thailand. This study aimed to determine the morphological variations of wings of An. minimus A and to clarify the specific status of An. minimus in Ban Khun Huay, Ban Pa Dae, and Ban Tham Seau, Mae Sot district, Tak Province, Thailand. Anopheline larvae were collected from the fields between October 2002 and September 2003, allowed to emerge into adults in the laboratory and identified by morphological and molecular characterization. About 1,715 of female An. minimus A were separated into 8 groups based on their wing scale patterns. Polymerase Chain Reaction Restriction Fragment Length Polymorphism (PCR-RFLP) assay (ribosomal DNA ITS2) confirmed the identification of An. minimus A in all 8 groups.


Sujets)
Animaux , Anopheles/anatomie et histologie , Amorces ADN , Femelle , Vecteurs insectes/anatomie et histologie , Larve , Réaction de polymérisation en chaîne , Spécificité d'espèce , Thaïlande , /anatomie et histologie
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